Viral and immunologic examination of human immunodeficiency virus type 1-infected, persistently seronegative persons

Dennis L. Ellenberger, Patrick S. Sullivan, Jonathan Dorn, Charles Schable, Thomas J. Spira, Thomas M. Folks, Renu B. Lal

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Persons who were human immunodeficiency virus type 1 (HIV-1)-infected but who remained persistently seronegative (HIPS) on HIV-1 antibody tests were examined through AIDS case surveillance. Six such individuals (HIPS-1 to -4, -7, and -9) were examined to determine whether their persistent seronegativity was attributable to immune dysfunction or infection with atypical HIV. Of the 6, 4 had antibody titers to at least 1 other common pathogen. In vitro stimulation of peripheral blood mononuclear cells from HIPS-4 and HIPS-7 with pokeweed mitogen or phosphorothioate oligodeoxynucleotide (direct B cell mitogen) did not produce HIV-1-specific antibody. Reconstitution experiments with recombinant interleukin (rIL)-4 and rIL-12 also had no impact on antibody production. Virus isolates from HIPS-4 and -9 were R5X4-tropic, whereas HIPS-7 was CCR5-tropic only. Sequence analysis of long terminal repeat, p24, and env gp41 did not reveal any specific mutation, and phylogenetic analysis confirmed that all 6 virus specimens were HIV-1 subtype B. These data suggest that the lack of a detectable antibody response in these patients may be the result of immune dysfunction.

Original languageEnglish (US)
Pages (from-to)1033-1042
Number of pages10
JournalJournal of Infectious Diseases
Volume180
Issue number4
DOIs
StatePublished - 1999
Externally publishedYes

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HIV-1
Antibody Formation
Antibodies
Viruses
Pokeweed Mitogens
Terminal Repeat Sequences
Oligodeoxyribonucleotides
Interleukin-12
Mitogens
Interleukin-4
Sequence Analysis
Blood Cells
Acquired Immunodeficiency Syndrome
B-Lymphocytes
HIV
Mutation
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Ellenberger, D. L., Sullivan, P. S., Dorn, J., Schable, C., Spira, T. J., Folks, T. M., & Lal, R. B. (1999). Viral and immunologic examination of human immunodeficiency virus type 1-infected, persistently seronegative persons. Journal of Infectious Diseases, 180(4), 1033-1042. https://doi.org/10.1086/315024

Viral and immunologic examination of human immunodeficiency virus type 1-infected, persistently seronegative persons. / Ellenberger, Dennis L.; Sullivan, Patrick S.; Dorn, Jonathan; Schable, Charles; Spira, Thomas J.; Folks, Thomas M.; Lal, Renu B.

In: Journal of Infectious Diseases, Vol. 180, No. 4, 1999, p. 1033-1042.

Research output: Contribution to journalArticle

Ellenberger, DL, Sullivan, PS, Dorn, J, Schable, C, Spira, TJ, Folks, TM & Lal, RB 1999, 'Viral and immunologic examination of human immunodeficiency virus type 1-infected, persistently seronegative persons', Journal of Infectious Diseases, vol. 180, no. 4, pp. 1033-1042. https://doi.org/10.1086/315024
Ellenberger, Dennis L. ; Sullivan, Patrick S. ; Dorn, Jonathan ; Schable, Charles ; Spira, Thomas J. ; Folks, Thomas M. ; Lal, Renu B. / Viral and immunologic examination of human immunodeficiency virus type 1-infected, persistently seronegative persons. In: Journal of Infectious Diseases. 1999 ; Vol. 180, No. 4. pp. 1033-1042.
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