TY - JOUR
T1 - VEGF analysis induced by endothelialized gas-plasma treated D,L-PLA scaffolds
AU - Polan, Jodie L.
AU - Morse, Brian
AU - Wetherold, Suzanne
AU - Villanueva-Vedia, Rosa E.
AU - Phelix, Clyde
AU - Barera-Roderiquiz, Edwin
AU - Waggoner, Douglas
AU - Goswami, Nilesh
AU - Munoz, Oscar
AU - Agrawal, C. Mauli
AU - Bailey, Steve R.
N1 - Funding Information:
We are grateful for the assistance of Eugene Sprague, PhD, and Holly White, BSc. This project was funded in part by the University of Texas Health Science Center at San Antonio, Institutional Review Grant to Dr. S.R. Bailey.
PY - 2002
Y1 - 2002
N2 - Purpose: Vascular endothelial growth factor (VEGF) isoforms play different roles in the temporal sprouting of endothelial-lined vessels in a nude mouse peritoneal model as cells respond to nontreated control and gas-plasma-treated bioresorbable poly-D,L-lactide acid 3D scaffolds with human aortic endothelial cells (HAEC). Methods and materials: Nude mice peritoneums were incubated with HAEC (CW=control; TW=gas-plasma treated) or polymer scaffolds (C p=control; Tp=treated) for 12, 24 and 72 days. Cytoplasmic and nuclear protein fractions were isolated using NER, electrophoresized using NuPAGE-MES and analyzed by WesternBreeze Chemiluminescent. Results: Prominent VEGF bands included 28, 45 and 62 kDa; 52-kDa VEGF observed in cytoplasmic TW fractions contributed about 18.6% at 12 days, 20.0% at 24 days and 13.1% at 72 days of the total VEGF signal. Yet, it was only noted in CW at 72 days where it accounted for 6.9%. A unique 32-kDa band appeared in both Cp (24.6%) and Tp (18.3%). Significant differences between band densities occurred for cytoplasmic nuclear CW24-TW24 (P=.022), CW72-TW72 (P=.011) and, also, cytoplasmic C p24-Tp24 (P=.038). Conclusions: The temporal and spatial organization of the TW isoforms results in more angiogenesis.
AB - Purpose: Vascular endothelial growth factor (VEGF) isoforms play different roles in the temporal sprouting of endothelial-lined vessels in a nude mouse peritoneal model as cells respond to nontreated control and gas-plasma-treated bioresorbable poly-D,L-lactide acid 3D scaffolds with human aortic endothelial cells (HAEC). Methods and materials: Nude mice peritoneums were incubated with HAEC (CW=control; TW=gas-plasma treated) or polymer scaffolds (C p=control; Tp=treated) for 12, 24 and 72 days. Cytoplasmic and nuclear protein fractions were isolated using NER, electrophoresized using NuPAGE-MES and analyzed by WesternBreeze Chemiluminescent. Results: Prominent VEGF bands included 28, 45 and 62 kDa; 52-kDa VEGF observed in cytoplasmic TW fractions contributed about 18.6% at 12 days, 20.0% at 24 days and 13.1% at 72 days of the total VEGF signal. Yet, it was only noted in CW at 72 days where it accounted for 6.9%. A unique 32-kDa band appeared in both Cp (24.6%) and Tp (18.3%). Significant differences between band densities occurred for cytoplasmic nuclear CW24-TW24 (P=.022), CW72-TW72 (P=.011) and, also, cytoplasmic C p24-Tp24 (P=.038). Conclusions: The temporal and spatial organization of the TW isoforms results in more angiogenesis.
KW - Angiogenesis
KW - Bioresorbable scaffolds
KW - Gas-plasma treatment
KW - Vascular endothelial growth factor
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U2 - 10.1016/S1522-1865(03)00100-8
DO - 10.1016/S1522-1865(03)00100-8
M3 - Article
C2 - 12974370
AN - SCOPUS:0142258758
VL - 3
SP - 176
EP - 182
JO - Cardiovascular Revascularization Medicine
JF - Cardiovascular Revascularization Medicine
SN - 1553-8389
IS - 3-4
ER -