VEGF analysis induced by endothelialized gas-plasma treated D,L-PLA scaffolds

Jodie L. Polan, Brian Morse, Suzanne Wetherold, Rosa E. Villanueva-Vedia, Clyde Phelix, Edwin Barera-Roderiquiz, Douglas Waggoner, Nilesh Goswami, Oscar Munoz, C. Mauli Agrawal, Steve R. Bailey

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Purpose: Vascular endothelial growth factor (VEGF) isoforms play different roles in the temporal sprouting of endothelial-lined vessels in a nude mouse peritoneal model as cells respond to nontreated control and gas-plasma-treated bioresorbable poly-D,L-lactide acid 3D scaffolds with human aortic endothelial cells (HAEC). Methods and materials: Nude mice peritoneums were incubated with HAEC (CW=control; TW=gas-plasma treated) or polymer scaffolds (C p=control; Tp=treated) for 12, 24 and 72 days. Cytoplasmic and nuclear protein fractions were isolated using NER, electrophoresized using NuPAGE-MES and analyzed by WesternBreeze Chemiluminescent. Results: Prominent VEGF bands included 28, 45 and 62 kDa; 52-kDa VEGF observed in cytoplasmic TW fractions contributed about 18.6% at 12 days, 20.0% at 24 days and 13.1% at 72 days of the total VEGF signal. Yet, it was only noted in CW at 72 days where it accounted for 6.9%. A unique 32-kDa band appeared in both Cp (24.6%) and Tp (18.3%). Significant differences between band densities occurred for cytoplasmic nuclear CW24-TW24 (P=.022), CW72-TW72 (P=.011) and, also, cytoplasmic C p24-Tp24 (P=.038). Conclusions: The temporal and spatial organization of the TW isoforms results in more angiogenesis.

Original languageEnglish (US)
Pages (from-to)176-182
Number of pages7
JournalCardiovascular Radiation Medicine
Volume3
Issue number3-4
DOIs
StatePublished - Jan 1 2002

Keywords

  • Angiogenesis
  • Bioresorbable scaffolds
  • Gas-plasma treatment
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Surgery
  • Molecular Medicine
  • Cardiology and Cardiovascular Medicine

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