TY - JOUR
T1 - VEGF analysis induced by endothelialized gas-plasma treated D,L-PLA scaffolds
AU - Polan, Jodie L.
AU - Morse, Brian
AU - Wetherold, Suzanne
AU - Villanueva-Vedia, Rosa E.
AU - Phelix, Clyde
AU - Barera-Roderiquiz, Edwin
AU - Waggoner, Douglas
AU - Goswami, Nilesh
AU - Munoz, Oscar
AU - Agrawal, C. Mauli
AU - Bailey, Steve R.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Purpose: Vascular endothelial growth factor (VEGF) isoforms play different roles in the temporal sprouting of endothelial-lined vessels in a nude mouse peritoneal model as cells respond to nontreated control and gas-plasma-treated bioresorbable poly-D,L-lactide acid 3D scaffolds with human aortic endothelial cells (HAEC). Methods and materials: Nude mice peritoneums were incubated with HAEC (CW=control; TW=gas-plasma treated) or polymer scaffolds (C p=control; Tp=treated) for 12, 24 and 72 days. Cytoplasmic and nuclear protein fractions were isolated using NER, electrophoresized using NuPAGE-MES and analyzed by WesternBreeze Chemiluminescent. Results: Prominent VEGF bands included 28, 45 and 62 kDa; 52-kDa VEGF observed in cytoplasmic TW fractions contributed about 18.6% at 12 days, 20.0% at 24 days and 13.1% at 72 days of the total VEGF signal. Yet, it was only noted in CW at 72 days where it accounted for 6.9%. A unique 32-kDa band appeared in both Cp (24.6%) and Tp (18.3%). Significant differences between band densities occurred for cytoplasmic nuclear CW24-TW24 (P=.022), CW72-TW72 (P=.011) and, also, cytoplasmic C p24-Tp24 (P=.038). Conclusions: The temporal and spatial organization of the TW isoforms results in more angiogenesis.
AB - Purpose: Vascular endothelial growth factor (VEGF) isoforms play different roles in the temporal sprouting of endothelial-lined vessels in a nude mouse peritoneal model as cells respond to nontreated control and gas-plasma-treated bioresorbable poly-D,L-lactide acid 3D scaffolds with human aortic endothelial cells (HAEC). Methods and materials: Nude mice peritoneums were incubated with HAEC (CW=control; TW=gas-plasma treated) or polymer scaffolds (C p=control; Tp=treated) for 12, 24 and 72 days. Cytoplasmic and nuclear protein fractions were isolated using NER, electrophoresized using NuPAGE-MES and analyzed by WesternBreeze Chemiluminescent. Results: Prominent VEGF bands included 28, 45 and 62 kDa; 52-kDa VEGF observed in cytoplasmic TW fractions contributed about 18.6% at 12 days, 20.0% at 24 days and 13.1% at 72 days of the total VEGF signal. Yet, it was only noted in CW at 72 days where it accounted for 6.9%. A unique 32-kDa band appeared in both Cp (24.6%) and Tp (18.3%). Significant differences between band densities occurred for cytoplasmic nuclear CW24-TW24 (P=.022), CW72-TW72 (P=.011) and, also, cytoplasmic C p24-Tp24 (P=.038). Conclusions: The temporal and spatial organization of the TW isoforms results in more angiogenesis.
KW - Angiogenesis
KW - Bioresorbable scaffolds
KW - Gas-plasma treatment
KW - Vascular endothelial growth factor
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U2 - 10.1016/S1522-1865(03)00100-8
DO - 10.1016/S1522-1865(03)00100-8
M3 - Article
C2 - 12974370
AN - SCOPUS:0142258758
VL - 3
SP - 176
EP - 182
JO - Cardiovascular Revascularization Medicine
JF - Cardiovascular Revascularization Medicine
SN - 1553-8389
IS - 3-4
ER -