TY - JOUR
T1 - Vasoactive Intestinal Peptide Promotes Corneal Allograft Survival
AU - Satitpitakul, Vannarut
AU - Sun, Zhongmou
AU - Suri, Kunal
AU - Amouzegar, Afsaneh
AU - Katikireddy, Kishore R.
AU - Jurkunas, Ula V.
AU - Kheirkhah, Ahmad
AU - Dana, Reza
N1 - Funding Information:
Supported in part by the Eye Bank Association of America (A.K.), Eversight grant award (A.K.), the New England Cornea Transplant Fund (A.K.), the Harvard Cornea Center of Excellence Fellowship (A.K.), and the Massachusetts Lions Club (A.K., U.V.J., and R.D.).
Publisher Copyright:
© 2018 American Society for Investigative Pathology
PY - 2018/9
Y1 - 2018/9
N2 - Corneal transplantation is the most prevalent form of tissue transplantation. The success of corneal transplantation mainly relies on the integrity of corneal endothelial cells (CEnCs), which maintain graft transparency. CEnC density decreases significantly after corneal transplantation even in the absence of graft rejection. To date, different strategies have been used to enhance CEnC survival. The neuropeptide vasoactive intestinal peptide (VIP) improves CEnC integrity during donor cornea tissue storage and protects CEnCs against oxidative stress–induced apoptosis. However, little is known about the effect of exogenous administration of VIP on corneal transplant outcomes. We found that VIP significantly accelerates endothelial wound closure and suppresses interferon-γ– and tumor necrosis factor-α–induced CEnC apoptosis in vitro in a dose-dependent manner. In addition, we found that intracameral administration of VIP to mice undergoing syngeneic corneal transplantation with endothelial injury increases CEnC density and decreases graft opacity scores. Finally, using a mouse model of allogeneic corneal transplantation, we found for the first time that treatment with VIP significantly suppresses posttransplantation CEnC loss and improves corneal allograft survival.
AB - Corneal transplantation is the most prevalent form of tissue transplantation. The success of corneal transplantation mainly relies on the integrity of corneal endothelial cells (CEnCs), which maintain graft transparency. CEnC density decreases significantly after corneal transplantation even in the absence of graft rejection. To date, different strategies have been used to enhance CEnC survival. The neuropeptide vasoactive intestinal peptide (VIP) improves CEnC integrity during donor cornea tissue storage and protects CEnCs against oxidative stress–induced apoptosis. However, little is known about the effect of exogenous administration of VIP on corneal transplant outcomes. We found that VIP significantly accelerates endothelial wound closure and suppresses interferon-γ– and tumor necrosis factor-α–induced CEnC apoptosis in vitro in a dose-dependent manner. In addition, we found that intracameral administration of VIP to mice undergoing syngeneic corneal transplantation with endothelial injury increases CEnC density and decreases graft opacity scores. Finally, using a mouse model of allogeneic corneal transplantation, we found for the first time that treatment with VIP significantly suppresses posttransplantation CEnC loss and improves corneal allograft survival.
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U2 - 10.1016/j.ajpath.2018.05.010
DO - 10.1016/j.ajpath.2018.05.010
M3 - Article
C2 - 30097165
AN - SCOPUS:85053815915
VL - 188
SP - 2016
EP - 2024
JO - American Journal of Pathology
JF - American Journal of Pathology
SN - 0002-9440
IS - 9
ER -