Vascular endottielial cell proliferation in culture and the influence of flow

M. J. Levesque, R. M. Nerem, E. A. Sprague

Research output: Contribution to journalArticlepeer-review

144 Scopus citations

Abstract

The influence of laminar shear stress on cell proliferation was investigated for subconfluent bovine aortic endothelial cell monolayers seeded on either glass or Thermanox®. The effect of both steady and pulsatile shear stress was studied. For bovine aortic endothelial cells on Thermanox® exposed to steady flow, shear stress levels > 15 dyne/cm2 resulted in a dose-related reduction in the rate of cell proliferation. At 90 dyne/cm2, the rate of proliferation was virtually totally arrested for 48 h, but then resumed. Pulsatile shear stress produced an exaggeration of the effect observed in response to steady shear stress. Bovine aortic endothelial cells seeded on glass, exhibited a similar but more sensitive response, with a significant reduction in growth rate observed after 24 h at shear stress levels > 5 dyne/cm2 and a near cessation of proliferation at 13 dyne/cm2.

Original languageEnglish (US)
Pages (from-to)702-707
Number of pages6
JournalBiomaterials
Volume11
Issue number9
DOIs
StatePublished - Nov 1990

Keywords

  • Cell growth
  • endothelium
  • vascular prostheses

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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