Vascular endottielial cell proliferation in culture and the influence of flow

M. J. Levesque; R. M. Nerem; E. A. Sprague

doi:10.1016/0142-9612(90)90031-K

Vascular endottielial cell proliferation in culture and the influence of flow

M. J. Levesque, R. M. Nerem, E. A. Sprague

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

The influence of laminar shear stress on cell proliferation was investigated for subconfluent bovine aortic endothelial cell monolayers seeded on either glass or Thermanox®. The effect of both steady and pulsatile shear stress was studied. For bovine aortic endothelial cells on Thermanox® exposed to steady flow, shear stress levels > 15 dyne/cm² resulted in a dose-related reduction in the rate of cell proliferation. At 90 dyne/cm², the rate of proliferation was virtually totally arrested for 48 h, but then resumed. Pulsatile shear stress produced an exaggeration of the effect observed in response to steady shear stress. Bovine aortic endothelial cells seeded on glass, exhibited a similar but more sensitive response, with a significant reduction in growth rate observed after 24 h at shear stress levels > 5 dyne/cm² and a near cessation of proliferation at 13 dyne/cm².

Original language	English (US)
Pages (from-to)	702-707
Number of pages	6
Journal	Biomaterials
Volume	11
Issue number	9
DOIs	https://doi.org/10.1016/0142-9612(90)90031-K
State	Published - Nov 1990
Externally published	Yes

Keywords

Cell growth
endothelium
vascular prostheses

ASJC Scopus subject areas

Mechanics of Materials
Ceramics and Composites
Bioengineering
Biophysics
Biomaterials

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10.1016/0142-9612(90)90031-K

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title = "Vascular endottielial cell proliferation in culture and the influence of flow",

abstract = "The influence of laminar shear stress on cell proliferation was investigated for subconfluent bovine aortic endothelial cell monolayers seeded on either glass or Thermanox{\textregistered}. The effect of both steady and pulsatile shear stress was studied. For bovine aortic endothelial cells on Thermanox{\textregistered} exposed to steady flow, shear stress levels > 15 dyne/cm2 resulted in a dose-related reduction in the rate of cell proliferation. At 90 dyne/cm2, the rate of proliferation was virtually totally arrested for 48 h, but then resumed. Pulsatile shear stress produced an exaggeration of the effect observed in response to steady shear stress. Bovine aortic endothelial cells seeded on glass, exhibited a similar but more sensitive response, with a significant reduction in growth rate observed after 24 h at shear stress levels > 5 dyne/cm2 and a near cessation of proliferation at 13 dyne/cm2.",

keywords = "Cell growth, endothelium, vascular prostheses",

author = "Levesque, {M. J.} and Nerem, {R. M.} and Sprague, {E. A.}",

note = "Funding Information: This research was supported by National Institutes of Health grants HL-26890 and HL-41 175 and by National Science Foundation grant EET-8815656. The authors also thank R.W. Alexander, P.R. Girard and C.J. Schwartz for the many discussions which have been held and their contributions to this work.",

year = "1990",

month = nov,

doi = "10.1016/0142-9612(90)90031-K",

language = "English (US)",

volume = "11",

pages = "702--707",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier Ltd",

number = "9",

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T1 - Vascular endottielial cell proliferation in culture and the influence of flow

AU - Levesque, M. J.

AU - Nerem, R. M.

AU - Sprague, E. A.

N1 - Funding Information: This research was supported by National Institutes of Health grants HL-26890 and HL-41 175 and by National Science Foundation grant EET-8815656. The authors also thank R.W. Alexander, P.R. Girard and C.J. Schwartz for the many discussions which have been held and their contributions to this work.

PY - 1990/11

Y1 - 1990/11

N2 - The influence of laminar shear stress on cell proliferation was investigated for subconfluent bovine aortic endothelial cell monolayers seeded on either glass or Thermanox®. The effect of both steady and pulsatile shear stress was studied. For bovine aortic endothelial cells on Thermanox® exposed to steady flow, shear stress levels > 15 dyne/cm2 resulted in a dose-related reduction in the rate of cell proliferation. At 90 dyne/cm2, the rate of proliferation was virtually totally arrested for 48 h, but then resumed. Pulsatile shear stress produced an exaggeration of the effect observed in response to steady shear stress. Bovine aortic endothelial cells seeded on glass, exhibited a similar but more sensitive response, with a significant reduction in growth rate observed after 24 h at shear stress levels > 5 dyne/cm2 and a near cessation of proliferation at 13 dyne/cm2.

AB - The influence of laminar shear stress on cell proliferation was investigated for subconfluent bovine aortic endothelial cell monolayers seeded on either glass or Thermanox®. The effect of both steady and pulsatile shear stress was studied. For bovine aortic endothelial cells on Thermanox® exposed to steady flow, shear stress levels > 15 dyne/cm2 resulted in a dose-related reduction in the rate of cell proliferation. At 90 dyne/cm2, the rate of proliferation was virtually totally arrested for 48 h, but then resumed. Pulsatile shear stress produced an exaggeration of the effect observed in response to steady shear stress. Bovine aortic endothelial cells seeded on glass, exhibited a similar but more sensitive response, with a significant reduction in growth rate observed after 24 h at shear stress levels > 5 dyne/cm2 and a near cessation of proliferation at 13 dyne/cm2.

KW - Cell growth

KW - endothelium

KW - vascular prostheses

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SN - 0142-9612

VL - 11

SP - 702

EP - 707

JO - Biomaterials

JF - Biomaterials

IS - 9

ER -

Vascular endottielial cell proliferation in culture and the influence of flow

Abstract

Keywords

ASJC Scopus subject areas

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