Validation of T2-based oxygen extraction fraction measurement with 15O positron emission tomography

Dengrong Jiang, Shengwen Deng, Crystal G. Franklin, Michael O'Boyle, Wei Zhang, Betty L. Heyl, Li Pan, Paul A. Jerabek, Peter T. Fox, Hanzhang Lu

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Purpose: To evaluate the accuracy of T2-based whole-brain oxygen extraction fraction (OEF) estimation by comparing it with gold standard 15O-PET measurements. Methods: Sixteen healthy adult subjects underwent MRI and 15O-PET OEF measurements on the same day. On MRI, whole-brain OEF was quantified by T2-relaxation-under-spin-tagging (TRUST) MRI, based on subject-specific hematocrit. The TRUST OEF was compared to the whole-brain averaged OEF produced by 15O-PET. Agreement between TRUST and 15O-PET whole-brain OEF measurements was examined in terms of intraclass correlation coefficient (ICC) and in absolute OEF values. In a subset of 10 subjects, test-retest reproducibility of whole-brain OEF was also evaluated and compared between the two modalities. Results: Across the 16 subjects, the mean whole-brain OEF of TRUST and 15O-PET were 36.44 ± 4.07% and 36.45 ± 3.65%, respectively, showing no difference between the two modalities (P =.99). TRUST whole-brain OEF strongly correlated with that of 15O-PET (N = 16, ICC = 0.90, P = 4 × 10−7). The coefficient-of-variation of TRUST and 15O-PET whole-brain OEF measurements were 1.79 ± 0.67% and 2.06 ± 1.55%, respectively, showing no difference between the two modalities (N = 10, P =.64). Further analyses on the effect of hematocrit revealed that correlation between PET OEF and TRUST OEF with assumed hematocrit remained significant (ICC = 0.8, P < 2 × 10−5). Conclusion: Whole-brain OEF measured by TRUST was in excellent agreement with gold standard 15O-PET, with highly comparable accuracy and reproducibility. These findings suggest that TRUST MRI can provide accurate quantification of whole-brain OEF noninvasively.

Original languageEnglish (US)
Pages (from-to)290-297
Number of pages8
JournalMagnetic Resonance in Medicine
Issue number1
StatePublished - Jan 1 2021


  • O PET
  • oxygen extraction fraction
  • oxygen saturation fraction
  • reproducibility
  • venous oxygenation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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