Validation of primed in situ labeling (PRINS) for interphase analysis: Comparative studies with conventional fluorescence in situ hybridization and chromosome analyses

Gopal Rao V.N. Velagaleti, Sugandhi A. Tharapel, Avirachan T. Tharapel

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Primed in situ labeling (PRINS) is a rapidly developing new technology with wide ranging clinical applications. To assess the sensitivity, specificity, and accuracy of PRINS, we carried out a retrospective study on cultured bone marrow cells to detect aneuploidy for chromosomes 7, 8, and 12. The results were then compared to the results of previous fluorescence in situ hybridization (FISH) and chromosome analyses (CA). In patients who showed aneuploidy with CA, both FISH and PRINS confirmed the aneuploidy in interphase cells, FISH and PRINS also showed excellent correlation with conventional cytogenetic analysis for the detection of mosaic aneuploidies. However, both FISH and PRINS showed significantly higher sensitivity in the detection of abnormal clones compared to CA. In 9 of the 17 cases, there were no significant differences in the detection rates between the two methods. Based on our studies, we conclude that PRINS is as sensitive as FISH in most cases for aneuploidy detection; and that PRINS, like FISH, is more sensitive than conventional CA for aneuploidy detection.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume108
Issue number2
DOIs
StatePublished - Jan 15 1999
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'Validation of primed in situ labeling (PRINS) for interphase analysis: Comparative studies with conventional fluorescence in situ hybridization and chromosome analyses'. Together they form a unique fingerprint.

Cite this