Validation of a specific radioimmunoassay to measure plasma cortisol in the fetal sheep

David M. Magyar; John E. Buster; Carlene W. Eisner; Peter W. Nathanielsz; Rene Oliveros; Guy E. Abraham

doi:10.1016/S0039-128X(79)80005-7

Validation of a specific radioimmunoassay to measure plasma cortisol in the fetal sheep

David M. Magyar, John E. Buster, Carlene W. Eisner, Peter W. Nathanielsz, Rene Oliveros, Guy E. Abraham

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A procedure utilizing co-chromatography and complementary antiserum comparisons was employed to assess the specificity of a cortisol radioimmunoassay for use in the chronically catheterized fetal sheep preparation. Complementary antiserum comparisons is a technique by which two different cortisol antisera, prepared from conjugates attached at opposite ends of the cortisol molecule, were used to determine cortisol concentrations in the same ovine fetal plasma specimens. Results were not significantly different between the two groups, each measured by a different antiserum. This procedure may be used to assess assay specificity in any species in which steroid radioimmunoassays are being adapted.

Original language	English (US)
Pages (from-to)	45-54
Number of pages	10
Journal	Steroids
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/S0039-128X(79)80005-7
State	Published - Jan 1979
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/S0039-128X(79)80005-7

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abstract = "A procedure utilizing co-chromatography and complementary antiserum comparisons was employed to assess the specificity of a cortisol radioimmunoassay for use in the chronically catheterized fetal sheep preparation. Complementary antiserum comparisons is a technique by which two different cortisol antisera, prepared from conjugates attached at opposite ends of the cortisol molecule, were used to determine cortisol concentrations in the same ovine fetal plasma specimens. Results were not significantly different between the two groups, each measured by a different antiserum. This procedure may be used to assess assay specificity in any species in which steroid radioimmunoassays are being adapted.",

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AU - Nathanielsz, Peter W.

AU - Oliveros, Rene

AU - Abraham, Guy E.

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AB - A procedure utilizing co-chromatography and complementary antiserum comparisons was employed to assess the specificity of a cortisol radioimmunoassay for use in the chronically catheterized fetal sheep preparation. Complementary antiserum comparisons is a technique by which two different cortisol antisera, prepared from conjugates attached at opposite ends of the cortisol molecule, were used to determine cortisol concentrations in the same ovine fetal plasma specimens. Results were not significantly different between the two groups, each measured by a different antiserum. This procedure may be used to assess assay specificity in any species in which steroid radioimmunoassays are being adapted.

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Validation of a specific radioimmunoassay to measure plasma cortisol in the fetal sheep

Abstract

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