Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice

Luis D. Giavedoni, Leslie Jones, Murray B. Gardner, Helen L. Gibson, Chun Ting Lee Ng, Philip J. Barr, Tilahun Yilma

    Research output: Contribution to journalArticle

    45 Scopus citations

    Abstract

    We have developed a method for attenuating vaccinia virus recombinants by expressing a fusion protein of a lymphokine and an immunogen. Chimeric genes were constructed that coded for γ Interferon (IFN-γ) and structural proteins of the human immunodeficiency virus type 1 (HIV-1). In this study, we describe the biological and immunological properties of vaccinia virus recombinants expressing chimeric genes of murine or human IFN-γ with glycoprotein gp120, gag, and a fragment of gp41. All fusion proteins retained the antigenic characteristics of both IFN-γ and HIV as shown by immunoblot analysis. However, the antiviral activity of IFN-γ could be demonstrated only for the IFN-γ-gag fusion protein. In contrast, the attenuating activity of IFN-γ for nude mice was retained by all of the recombinants, albeit at various rates. Unlike the antiviral activity, the attenuating activity of IFN-γ was not species specific. Implications for the development of attenuated live recombinant vaccines for AIDS are discussed.

    Original languageEnglish (US)
    Pages (from-to)3409-3413
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume89
    Issue number8
    DOIs
    StatePublished - Jan 1 1992

    Keywords

    • Chimeric proteins
    • Lymphokines
    • Vaccine development

    ASJC Scopus subject areas

    • General

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