Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice

Luis D. Giavedoni; Leslie Jones; Murray B. Gardner; Helen L. Gibson; Chun Ting Lee Ng; Philip J. Barr; Tilahun Yilma

doi:10.1073/pnas.89.8.3409

Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice

Luis D. Giavedoni, Leslie Jones, Murray B. Gardner, Helen L. Gibson, Chun Ting Lee Ng, Philip J. Barr, Tilahun Yilma

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

We have developed a method for attenuating vaccinia virus recombinants by expressing a fusion protein of a lymphokine and an immunogen. Chimeric genes were constructed that coded for γ Interferon (IFN-γ) and structural proteins of the human immunodeficiency virus type 1 (HIV-1). In this study, we describe the biological and immunological properties of vaccinia virus recombinants expressing chimeric genes of murine or human IFN-γ with glycoprotein gp120, gag, and a fragment of gp41. All fusion proteins retained the antigenic characteristics of both IFN-γ and HIV as shown by immunoblot analysis. However, the antiviral activity of IFN-γ could be demonstrated only for the IFN-γ-gag fusion protein. In contrast, the attenuating activity of IFN-γ for nude mice was retained by all of the recombinants, albeit at various rates. Unlike the antiviral activity, the attenuating activity of IFN-γ was not species specific. Implications for the development of attenuated live recombinant vaccines for AIDS are discussed.

Original language	English (US)
Pages (from-to)	3409-3413
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	8
DOIs	https://doi.org/10.1073/pnas.89.8.3409
State	Published - 1992

Keywords

Chimeric proteins
Lymphokines
Vaccine development

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.89.8.3409

Cite this

Giavedoni, L. D., Jones, L., Gardner, M. B., Gibson, H. L., Lee Ng, C. T., Barr, P. J., & Yilma, T. (1992). Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice. Proceedings of the National Academy of Sciences of the United States of America, 89(8), 3409-3413. https://doi.org/10.1073/pnas.89.8.3409

Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice. / Giavedoni, Luis D.; Jones, Leslie; Gardner, Murray B.; Gibson, Helen L.; Lee Ng, Chun Ting; Barr, Philip J.; Yilma, Tilahun.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 8, 1992, p. 3409-3413.

Research output: Contribution to journal › Article › peer-review

Giavedoni, LD, Jones, L, Gardner, MB, Gibson, HL, Lee Ng, CT, Barr, PJ & Yilma, T 1992, 'Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 8, pp. 3409-3413. https://doi.org/10.1073/pnas.89.8.3409

Giavedoni, Luis D. ; Jones, Leslie ; Gardner, Murray B. ; Gibson, Helen L. ; Lee Ng, Chun Ting ; Barr, Philip J. ; Yilma, Tilahun. / Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 8. pp. 3409-3413.

@article{702cb9fef9af4f77afa98d7e43aa0041,

title = "Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice",

abstract = "We have developed a method for attenuating vaccinia virus recombinants by expressing a fusion protein of a lymphokine and an immunogen. Chimeric genes were constructed that coded for γ Interferon (IFN-γ) and structural proteins of the human immunodeficiency virus type 1 (HIV-1). In this study, we describe the biological and immunological properties of vaccinia virus recombinants expressing chimeric genes of murine or human IFN-γ with glycoprotein gp120, gag, and a fragment of gp41. All fusion proteins retained the antigenic characteristics of both IFN-γ and HIV as shown by immunoblot analysis. However, the antiviral activity of IFN-γ could be demonstrated only for the IFN-γ-gag fusion protein. In contrast, the attenuating activity of IFN-γ for nude mice was retained by all of the recombinants, albeit at various rates. Unlike the antiviral activity, the attenuating activity of IFN-γ was not species specific. Implications for the development of attenuated live recombinant vaccines for AIDS are discussed.",

keywords = "Chimeric proteins, Lymphokines, Vaccine development",

author = "Giavedoni, {Luis D.} and Leslie Jones and Gardner, {Murray B.} and Gibson, {Helen L.} and {Lee Ng}, {Chun Ting} and Barr, {Philip J.} and Tilahun Yilma",

year = "1992",

doi = "10.1073/pnas.89.8.3409",

language = "English (US)",

volume = "89",

pages = "3409--3413",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "8",

}

TY - JOUR

T1 - Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice

AU - Giavedoni, Luis D.

AU - Jones, Leslie

AU - Gardner, Murray B.

AU - Gibson, Helen L.

AU - Lee Ng, Chun Ting

AU - Barr, Philip J.

AU - Yilma, Tilahun

PY - 1992

Y1 - 1992

N2 - We have developed a method for attenuating vaccinia virus recombinants by expressing a fusion protein of a lymphokine and an immunogen. Chimeric genes were constructed that coded for γ Interferon (IFN-γ) and structural proteins of the human immunodeficiency virus type 1 (HIV-1). In this study, we describe the biological and immunological properties of vaccinia virus recombinants expressing chimeric genes of murine or human IFN-γ with glycoprotein gp120, gag, and a fragment of gp41. All fusion proteins retained the antigenic characteristics of both IFN-γ and HIV as shown by immunoblot analysis. However, the antiviral activity of IFN-γ could be demonstrated only for the IFN-γ-gag fusion protein. In contrast, the attenuating activity of IFN-γ for nude mice was retained by all of the recombinants, albeit at various rates. Unlike the antiviral activity, the attenuating activity of IFN-γ was not species specific. Implications for the development of attenuated live recombinant vaccines for AIDS are discussed.

AB - We have developed a method for attenuating vaccinia virus recombinants by expressing a fusion protein of a lymphokine and an immunogen. Chimeric genes were constructed that coded for γ Interferon (IFN-γ) and structural proteins of the human immunodeficiency virus type 1 (HIV-1). In this study, we describe the biological and immunological properties of vaccinia virus recombinants expressing chimeric genes of murine or human IFN-γ with glycoprotein gp120, gag, and a fragment of gp41. All fusion proteins retained the antigenic characteristics of both IFN-γ and HIV as shown by immunoblot analysis. However, the antiviral activity of IFN-γ could be demonstrated only for the IFN-γ-gag fusion protein. In contrast, the attenuating activity of IFN-γ for nude mice was retained by all of the recombinants, albeit at various rates. Unlike the antiviral activity, the attenuating activity of IFN-γ was not species specific. Implications for the development of attenuated live recombinant vaccines for AIDS are discussed.

KW - Chimeric proteins

KW - Lymphokines

KW - Vaccine development

UR - http://www.scopus.com/inward/record.url?scp=0026586948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026586948&partnerID=8YFLogxK

U2 - 10.1073/pnas.89.8.3409

DO - 10.1073/pnas.89.8.3409

M3 - Article

C2 - 1565633

AN - SCOPUS:0026586948

VL - 89

SP - 3409

EP - 3413

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 8

ER -

Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this