We have developed a method for attenuating vaccinia virus recombinants by expressing a fusion protein of a lymphokine and an immunogen. Chimeric genes were constructed that coded for γ Interferon (IFN-γ) and structural proteins of the human immunodeficiency virus type 1 (HIV-1). In this study, we describe the biological and immunological properties of vaccinia virus recombinants expressing chimeric genes of murine or human IFN-γ with glycoprotein gp120, gag, and a fragment of gp41. All fusion proteins retained the antigenic characteristics of both IFN-γ and HIV as shown by immunoblot analysis. However, the antiviral activity of IFN-γ could be demonstrated only for the IFN-γ-gag fusion protein. In contrast, the attenuating activity of IFN-γ for nude mice was retained by all of the recombinants, albeit at various rates. Unlike the antiviral activity, the attenuating activity of IFN-γ was not species specific. Implications for the development of attenuated live recombinant vaccines for AIDS are discussed.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Jan 1 1992|
- Chimeric proteins
- Vaccine development
ASJC Scopus subject areas