Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice

Luis D. Giavedoni; Leslie Jones; Murray B. Gardner; Helen L. Gibson; Chun Ting Lee Ng; Philip J. Barr; Tilahun Yilma

doi:10.1073/pnas.89.8.3409

Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice

Luis D. Giavedoni, Leslie Jones, Murray B. Gardner, Helen L. Gibson, Chun Ting Lee Ng, Philip J. Barr, Tilahun Yilma

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

We have developed a method for attenuating vaccinia virus recombinants by expressing a fusion protein of a lymphokine and an immunogen. Chimeric genes were constructed that coded for γ Interferon (IFN-γ) and structural proteins of the human immunodeficiency virus type 1 (HIV-1). In this study, we describe the biological and immunological properties of vaccinia virus recombinants expressing chimeric genes of murine or human IFN-γ with glycoprotein gp120, gag, and a fragment of gp41. All fusion proteins retained the antigenic characteristics of both IFN-γ and HIV as shown by immunoblot analysis. However, the antiviral activity of IFN-γ could be demonstrated only for the IFN-γ-gag fusion protein. In contrast, the attenuating activity of IFN-γ for nude mice was retained by all of the recombinants, albeit at various rates. Unlike the antiviral activity, the attenuating activity of IFN-γ was not species specific. Implications for the development of attenuated live recombinant vaccines for AIDS are discussed.

Original language	English (US)
Pages (from-to)	3409-3413
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	8
DOIs	https://doi.org/10.1073/pnas.89.8.3409
State	Published - 1992

Keywords

Chimeric proteins
Lymphokines
Vaccine development

ASJC Scopus subject areas

General

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Giavedoni, L. D., Jones, L., Gardner, M. B., Gibson, H. L., Lee Ng, C. T., Barr, P. J., & Yilma, T. (1992). Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice. Proceedings of the National Academy of Sciences of the United States of America, 89(8), 3409-3413. https://doi.org/10.1073/pnas.89.8.3409

Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice. / Giavedoni, Luis D.; Jones, Leslie; Gardner, Murray B.; Gibson, Helen L.; Lee Ng, Chun Ting; Barr, Philip J.; Yilma, Tilahun.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 8, 1992, p. 3409-3413.

Research output: Contribution to journal › Article › peer-review

Giavedoni, LD, Jones, L, Gardner, MB, Gibson, HL, Lee Ng, CT, Barr, PJ & Yilma, T 1992, 'Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 8, pp. 3409-3413. https://doi.org/10.1073/pnas.89.8.3409

Giavedoni, Luis D. ; Jones, Leslie ; Gardner, Murray B. ; Gibson, Helen L. ; Lee Ng, Chun Ting ; Barr, Philip J. ; Yilma, Tilahun. / Vaccinia virus recombinants expressing chimeric proteins of human Immunodeficiency virus and γ interferon are attenuated for nude mice. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 8. pp. 3409-3413.

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