Vaccinia virus K1L protein supports viral replication in human and rabbit cells through a cell-type-specific set of its ankyrin repeat residues that are distinct from its binding site for ACAP2

Xiangzhi Meng, Yan Xiang

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Vaccinia virus (VV) K1L is a host-range gene and encodes a protein comprised of six ankyrin repeats (ANKs). We showed here that a large portion of the K1L protein, except ankyrin repeat 1 (ANK1) and C-terminal halves of ANK2 and ANK3, can be deleted or substituted with an unrelated ANK with no adverse effect on VV replication in human HeLa cells. In contrast, only ANK4 and ANK6 can be mutated without impairing VV replication in rabbit RK13 cells. The growth rate of VV in HeLa cells was reduced differentially by substituting phenylalanine 82 or serine 83 of ANK2 and abolished completely by substituting both residues. These substitutions, however, did not affect K1L's ability to bind ACAP2, a GTPase-activating protein for ARF6. Our data support the hypothesis that surface residues of a few consecutive K1L ANKs mediate the host-range function by interacting with protein factors that are distinct from ACAP2.

Original languageEnglish (US)
Pages (from-to)220-233
Number of pages14
JournalVirology
Volume353
Issue number1
DOIs
Publication statusPublished - Sep 15 2006

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Keywords

  • ACAP2
  • Ankyrin repeat
  • C7L
  • Host-range
  • K1L
  • Poxvirus
  • Vaccinia virus

ASJC Scopus subject areas

  • Virology

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