Vaccine-mediated immune responses to experimental pulmonary cryptococcus gattii infection in mice

Ashok K. Chaturvedi, Rumanasma S. Hameed, Karen L. Wozniak, Camaron R. Hole, Chrissy M Leopold Wager, Susan E Weintraub, Jose L. Lopez-Ribot, Floyd L. Wormley

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cryptococcus gattii is a fungal pathogen that can cause life-threatening respiratory and disseminated infections in immune-competent and immune-suppressed individuals. Currently, there are no standardized vaccines against cryptococcosis in humans, underlying an urgent need for effective therapies and/or vaccines. In this study, we evaluated the efficacy of intranasal immunization with C. gattii cell wall associated (CW) and/or cytoplasmic (CP) protein preparations to induce protection against experimental pulmonary C. gattii infection in mice. BALB/c mice immunized with C. gattii CW and/or CP protein preparations exhibited a significant reduction in pulmonary fungal burden and prolonged survival following pulmonary challenge with C. gattii. Protection was associated with significantly increased pro-inflammatory and Th1-type cytokine recall responses, in vitro and increased C. gattii-specific antibody production in immunized mice challenged with C. gattii. A number of immunodominant proteins were identified following immunoblot analysis of C. gattii CW and CP protein preparations using sera from immunized mice. Immunization with a combined CW and CP protein preparation resulted in an early increase in pulmonary T cell infiltrates following challenge with C. gattii. Overall, our studies show that C. gattii CW and CP protein preparations contain antigens that may be included in a subunit vaccine to induce prolonged protection against pulmonary C. gattii infection.

Original languageEnglish (US)
Article numbere104316
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 13 2014

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Cryptococcus gattii
Vaccines
lungs
immune response
vaccines
Lung
mice
Infection
infection
Immunization
Cell Wall
Proteins
cell walls
proteins
Subunit Vaccines
T-cells
Pathogens
immunization
Cells
Active Immunotherapy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chaturvedi, A. K., Hameed, R. S., Wozniak, K. L., Hole, C. R., Wager, C. M. L., Weintraub, S. E., ... Wormley, F. L. (2014). Vaccine-mediated immune responses to experimental pulmonary cryptococcus gattii infection in mice. PLoS One, 9(8), [e104316]. https://doi.org/10.1371/journal.pone.0104316

Vaccine-mediated immune responses to experimental pulmonary cryptococcus gattii infection in mice. / Chaturvedi, Ashok K.; Hameed, Rumanasma S.; Wozniak, Karen L.; Hole, Camaron R.; Wager, Chrissy M Leopold; Weintraub, Susan E; Lopez-Ribot, Jose L.; Wormley, Floyd L.

In: PLoS One, Vol. 9, No. 8, e104316, 13.08.2014.

Research output: Contribution to journalArticle

Chaturvedi, AK, Hameed, RS, Wozniak, KL, Hole, CR, Wager, CML, Weintraub, SE, Lopez-Ribot, JL & Wormley, FL 2014, 'Vaccine-mediated immune responses to experimental pulmonary cryptococcus gattii infection in mice', PLoS One, vol. 9, no. 8, e104316. https://doi.org/10.1371/journal.pone.0104316
Chaturvedi, Ashok K. ; Hameed, Rumanasma S. ; Wozniak, Karen L. ; Hole, Camaron R. ; Wager, Chrissy M Leopold ; Weintraub, Susan E ; Lopez-Ribot, Jose L. ; Wormley, Floyd L. / Vaccine-mediated immune responses to experimental pulmonary cryptococcus gattii infection in mice. In: PLoS One. 2014 ; Vol. 9, No. 8.
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