Utilization of MO6-G3 immortalized odontoblast cells in studies regarding dentinogenesis.

M. MacDougall, A. Unterbrink, D. Carnes, Sheela R Kadapakkam, X. Luan, Shuo Chen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tooth formation is the result of reciprocal instructive interactions between oral epithelium and cranial neural-crest-derived ectomesenchymal tissues. These interactions lead to the cytodifferentiation of highly specialized matrix-forming cell types, the ameloblast, odontoblast, and cementoblast, that produce the mineralized tissues enamel, dentin, and cementum, respectively. Our laboratory has been developing immortalized dental cell lines representative of these various cell types to facilitate studies on gene regulation, cell differentiation, matrix formation, and mineralization. Odontoblasts are solely responsible for the synthesis and secretion of the dentin extracellular matrix bilayer that consists of non-mineralized predentin and mineralized dentin. The mouse immortalized MO6-G3 cell line expresses the major matrix proteins associated with the odontoblast phenotype, producing a matrix that is capable of mineralization. This cell line serves as a useful tool in studies designed to explore the various processes of dentinogenesis. In this paper, we present studies using the mouse odontoblast cell line MO6-G3 as examples of the various research applications. Studies highlighted are: in vitro promoter studies investigating the tooth-specific gene regulation of the major non-collagenous dentin matrix protein, dentin sialophosphoprotein; regulation of tertiary dentin formation by cytokines, such as transforming growth factor-Beta 1; and the utilization of dentally relevant cells in dental material biocompatibility testing.

Original languageEnglish (US)
Pages (from-to)25-29
Number of pages5
JournalAdvances in dental research
Volume15
StatePublished - 2001

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Dentinogenesis
Odontoblasts
Dentin
Materials Testing
Dental Cementum
Cell Line
Tooth
Ameloblasts
Dental Materials
Neural Crest
Dental Enamel
Transforming Growth Factor beta
Genes
Extracellular Matrix
Cell Differentiation
Epithelium
Cytokines
Phenotype
Research
Proteins

Cite this

MacDougall, M., Unterbrink, A., Carnes, D., Kadapakkam, S. R., Luan, X., & Chen, S. (2001). Utilization of MO6-G3 immortalized odontoblast cells in studies regarding dentinogenesis. Advances in dental research, 15, 25-29.

Utilization of MO6-G3 immortalized odontoblast cells in studies regarding dentinogenesis. / MacDougall, M.; Unterbrink, A.; Carnes, D.; Kadapakkam, Sheela R; Luan, X.; Chen, Shuo.

In: Advances in dental research, Vol. 15, 2001, p. 25-29.

Research output: Contribution to journalArticle

MacDougall, M, Unterbrink, A, Carnes, D, Kadapakkam, SR, Luan, X & Chen, S 2001, 'Utilization of MO6-G3 immortalized odontoblast cells in studies regarding dentinogenesis.', Advances in dental research, vol. 15, pp. 25-29.
MacDougall, M. ; Unterbrink, A. ; Carnes, D. ; Kadapakkam, Sheela R ; Luan, X. ; Chen, Shuo. / Utilization of MO6-G3 immortalized odontoblast cells in studies regarding dentinogenesis. In: Advances in dental research. 2001 ; Vol. 15. pp. 25-29.
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