Using Operant Reach Chambers to Assess Mouse Skilled Forelimb Use After Stroke

Dene Betz; April M. Becker; Katherine M. Cotter; Andrew M. Sloan; Ann M. Stowe; Mark P. Goldberg

doi:10.1007/978-1-0716-2926-0_22

Using Operant Reach Chambers to Assess Mouse Skilled Forelimb Use After Stroke

Dene Betz, April M. Becker, Katherine M. Cotter, Andrew M. Sloan, Ann M. Stowe, Mark P. Goldberg

Research output: Contribution to journal › Article › peer-review

Abstract

Skilled forelimb reaching and grasping are important components of rodent motor performance. The isometric pull task can serve as a tool for quantifying forelimb function following stroke or other CNS injury as well as in forelimb rehabilitation. This task has been extensively developed for use in rats. Here, we describe methods of setup and training of an operant reach chamber for mice. Using a reward of peanut oil, mice are adaptively trained to pull a handle positioned slightly outside of an operant chamber, with automated recording of the number of attempts, force generated, success rate, and latency to maximal force.

Original language	English (US)
Pages (from-to)	327-343
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2616
DOIs	https://doi.org/10.1007/978-1-0716-2926-0_22
State	Published - 2023

Keywords

Forelimb function
Isometric pull task
Motor Impairment
Operant reach chambers
Stroke

ASJC Scopus subject areas

Genetics
Molecular Biology

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10.1007/978-1-0716-2926-0_22

Cite this

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title = "Using Operant Reach Chambers to Assess Mouse Skilled Forelimb Use After Stroke",

abstract = "Skilled forelimb reaching and grasping are important components of rodent motor performance. The isometric pull task can serve as a tool for quantifying forelimb function following stroke or other CNS injury as well as in forelimb rehabilitation. This task has been extensively developed for use in rats. Here, we describe methods of setup and training of an operant reach chamber for mice. Using a reward of peanut oil, mice are adaptively trained to pull a handle positioned slightly outside of an operant chamber, with automated recording of the number of attempts, force generated, success rate, and latency to maximal force.",

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AU - Becker, April M.

AU - Cotter, Katherine M.

AU - Sloan, Andrew M.

AU - Stowe, Ann M.

AU - Goldberg, Mark P.

PY - 2023

Y1 - 2023

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AB - Skilled forelimb reaching and grasping are important components of rodent motor performance. The isometric pull task can serve as a tool for quantifying forelimb function following stroke or other CNS injury as well as in forelimb rehabilitation. This task has been extensively developed for use in rats. Here, we describe methods of setup and training of an operant reach chamber for mice. Using a reward of peanut oil, mice are adaptively trained to pull a handle positioned slightly outside of an operant chamber, with automated recording of the number of attempts, force generated, success rate, and latency to maximal force.

KW - Forelimb function

KW - Isometric pull task

KW - Motor Impairment

KW - Operant reach chambers

KW - Stroke

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Using Operant Reach Chambers to Assess Mouse Skilled Forelimb Use After Stroke

Abstract

Keywords

ASJC Scopus subject areas

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