Use of 99mTc-labeled liposomes encapsulating blue dye for identification of the sentinel lymph node

William T Phillips, R. Klipper, B. Goins

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Colloidal radiopharmaceuticals are commonly used in combination with blue dye for localization of the sentinel node. Liposomes are colloidal particles composed of spontaneously forming lipid spheres that can carry a wide variety of diagnostic and therapeutic agents. Conventional liposomes are poorly retained in lymph nodes (<2% of the subcutaneously injected dose). We have previously described a system for increasing the retention of liposomes in the lymph nodes by ∼7-fold. This system is comprised of subcutaneously injected biotin-coated liposomes, followed by an adjacent injection of avidin. When the avidin moves into the lymphatic vessels, it causes aggregation of the biotin-coated liposomes that are also in the process of migrating through the lymphatic vessels. These aggregated liposomes become entrapped in the next encountered lymph node. In this study, we use this novel lymph node delivery system with liposomes that encapsulate blue dye, resulting in intense blue staining of the sentinel node. These liposomes can also be labeled with 99mTc, permitting scintigraphic imaging and radioguided probe localization of the sentinel node. Methods: Liposomes coated with biotin and coencapsulating blue dye and glutathione were labeled with 99mTc using hexamethylpropyleneamine oxime. Rabbits were subcutaneously administered 0.3 mL 99mTc-biotin-liposomes containing blue dye in both hind feet, followed by a subcutaneous injection (0.3 mL) of 5 mg avidin in only one hind foot (experimental). The other hind foot served as a control. Results: Labeling efficiencies (mean ± SEM) for liposomes encapsulating blue dye were 92.1% ± 1.9%. Necropsy at 24 h revealed that the popliteal node on the experimental leg receiving the avidin was intensely blue stained compared with virtually no blue coloration of the control node. Tissue counts of these nodes were 12.2 ± 1.5 percentage injected dose (%ID) in the experimental node compared with 1.2 ± 0.1 %ID in the control nodes (P < 0.0001). Conclusion: Biotin-liposomes encapsulating blue dye can be successfully labeled with 99mTc, providing a convenient option for the visualization and radiolocalization of the sentinel node. This biotin-liposome/avidin system may also have potential for the delivery of therapeutic drugs and radiopharmaceuticals to lymph nodes.

Original languageEnglish (US)
Pages (from-to)446-451
Number of pages6
JournalJournal of Nuclear Medicine
Volume42
Issue number3
StatePublished - 2001

Fingerprint

Liposomes
Coloring Agents
Biotin
Avidin
Lymph Nodes
Foot
Lymphatic Vessels
Radiopharmaceuticals
Sentinel Lymph Node
Subcutaneous Injections
Glutathione
Leg
Staining and Labeling
Rabbits
Lipids

Keywords

  • TC
  • Avidin
  • Blue dye
  • Liposomes
  • Sentinel node

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Use of 99mTc-labeled liposomes encapsulating blue dye for identification of the sentinel lymph node. / Phillips, William T; Klipper, R.; Goins, B.

In: Journal of Nuclear Medicine, Vol. 42, No. 3, 2001, p. 446-451.

Research output: Contribution to journalArticle

Phillips, William T ; Klipper, R. ; Goins, B. / Use of 99mTc-labeled liposomes encapsulating blue dye for identification of the sentinel lymph node. In: Journal of Nuclear Medicine. 2001 ; Vol. 42, No. 3. pp. 446-451.
@article{b5518a160b42400abacd180385a4d307,
title = "Use of 99mTc-labeled liposomes encapsulating blue dye for identification of the sentinel lymph node",
abstract = "Colloidal radiopharmaceuticals are commonly used in combination with blue dye for localization of the sentinel node. Liposomes are colloidal particles composed of spontaneously forming lipid spheres that can carry a wide variety of diagnostic and therapeutic agents. Conventional liposomes are poorly retained in lymph nodes (<2{\%} of the subcutaneously injected dose). We have previously described a system for increasing the retention of liposomes in the lymph nodes by ∼7-fold. This system is comprised of subcutaneously injected biotin-coated liposomes, followed by an adjacent injection of avidin. When the avidin moves into the lymphatic vessels, it causes aggregation of the biotin-coated liposomes that are also in the process of migrating through the lymphatic vessels. These aggregated liposomes become entrapped in the next encountered lymph node. In this study, we use this novel lymph node delivery system with liposomes that encapsulate blue dye, resulting in intense blue staining of the sentinel node. These liposomes can also be labeled with 99mTc, permitting scintigraphic imaging and radioguided probe localization of the sentinel node. Methods: Liposomes coated with biotin and coencapsulating blue dye and glutathione were labeled with 99mTc using hexamethylpropyleneamine oxime. Rabbits were subcutaneously administered 0.3 mL 99mTc-biotin-liposomes containing blue dye in both hind feet, followed by a subcutaneous injection (0.3 mL) of 5 mg avidin in only one hind foot (experimental). The other hind foot served as a control. Results: Labeling efficiencies (mean ± SEM) for liposomes encapsulating blue dye were 92.1{\%} ± 1.9{\%}. Necropsy at 24 h revealed that the popliteal node on the experimental leg receiving the avidin was intensely blue stained compared with virtually no blue coloration of the control node. Tissue counts of these nodes were 12.2 ± 1.5 percentage injected dose ({\%}ID) in the experimental node compared with 1.2 ± 0.1 {\%}ID in the control nodes (P < 0.0001). Conclusion: Biotin-liposomes encapsulating blue dye can be successfully labeled with 99mTc, providing a convenient option for the visualization and radiolocalization of the sentinel node. This biotin-liposome/avidin system may also have potential for the delivery of therapeutic drugs and radiopharmaceuticals to lymph nodes.",
keywords = "TC, Avidin, Blue dye, Liposomes, Sentinel node",
author = "Phillips, {William T} and R. Klipper and B. Goins",
year = "2001",
language = "English (US)",
volume = "42",
pages = "446--451",
journal = "Journal of Nuclear Medicine",
issn = "0161-5505",
publisher = "Society of Nuclear Medicine Inc.",
number = "3",

}

TY - JOUR

T1 - Use of 99mTc-labeled liposomes encapsulating blue dye for identification of the sentinel lymph node

AU - Phillips, William T

AU - Klipper, R.

AU - Goins, B.

PY - 2001

Y1 - 2001

N2 - Colloidal radiopharmaceuticals are commonly used in combination with blue dye for localization of the sentinel node. Liposomes are colloidal particles composed of spontaneously forming lipid spheres that can carry a wide variety of diagnostic and therapeutic agents. Conventional liposomes are poorly retained in lymph nodes (<2% of the subcutaneously injected dose). We have previously described a system for increasing the retention of liposomes in the lymph nodes by ∼7-fold. This system is comprised of subcutaneously injected biotin-coated liposomes, followed by an adjacent injection of avidin. When the avidin moves into the lymphatic vessels, it causes aggregation of the biotin-coated liposomes that are also in the process of migrating through the lymphatic vessels. These aggregated liposomes become entrapped in the next encountered lymph node. In this study, we use this novel lymph node delivery system with liposomes that encapsulate blue dye, resulting in intense blue staining of the sentinel node. These liposomes can also be labeled with 99mTc, permitting scintigraphic imaging and radioguided probe localization of the sentinel node. Methods: Liposomes coated with biotin and coencapsulating blue dye and glutathione were labeled with 99mTc using hexamethylpropyleneamine oxime. Rabbits were subcutaneously administered 0.3 mL 99mTc-biotin-liposomes containing blue dye in both hind feet, followed by a subcutaneous injection (0.3 mL) of 5 mg avidin in only one hind foot (experimental). The other hind foot served as a control. Results: Labeling efficiencies (mean ± SEM) for liposomes encapsulating blue dye were 92.1% ± 1.9%. Necropsy at 24 h revealed that the popliteal node on the experimental leg receiving the avidin was intensely blue stained compared with virtually no blue coloration of the control node. Tissue counts of these nodes were 12.2 ± 1.5 percentage injected dose (%ID) in the experimental node compared with 1.2 ± 0.1 %ID in the control nodes (P < 0.0001). Conclusion: Biotin-liposomes encapsulating blue dye can be successfully labeled with 99mTc, providing a convenient option for the visualization and radiolocalization of the sentinel node. This biotin-liposome/avidin system may also have potential for the delivery of therapeutic drugs and radiopharmaceuticals to lymph nodes.

AB - Colloidal radiopharmaceuticals are commonly used in combination with blue dye for localization of the sentinel node. Liposomes are colloidal particles composed of spontaneously forming lipid spheres that can carry a wide variety of diagnostic and therapeutic agents. Conventional liposomes are poorly retained in lymph nodes (<2% of the subcutaneously injected dose). We have previously described a system for increasing the retention of liposomes in the lymph nodes by ∼7-fold. This system is comprised of subcutaneously injected biotin-coated liposomes, followed by an adjacent injection of avidin. When the avidin moves into the lymphatic vessels, it causes aggregation of the biotin-coated liposomes that are also in the process of migrating through the lymphatic vessels. These aggregated liposomes become entrapped in the next encountered lymph node. In this study, we use this novel lymph node delivery system with liposomes that encapsulate blue dye, resulting in intense blue staining of the sentinel node. These liposomes can also be labeled with 99mTc, permitting scintigraphic imaging and radioguided probe localization of the sentinel node. Methods: Liposomes coated with biotin and coencapsulating blue dye and glutathione were labeled with 99mTc using hexamethylpropyleneamine oxime. Rabbits were subcutaneously administered 0.3 mL 99mTc-biotin-liposomes containing blue dye in both hind feet, followed by a subcutaneous injection (0.3 mL) of 5 mg avidin in only one hind foot (experimental). The other hind foot served as a control. Results: Labeling efficiencies (mean ± SEM) for liposomes encapsulating blue dye were 92.1% ± 1.9%. Necropsy at 24 h revealed that the popliteal node on the experimental leg receiving the avidin was intensely blue stained compared with virtually no blue coloration of the control node. Tissue counts of these nodes were 12.2 ± 1.5 percentage injected dose (%ID) in the experimental node compared with 1.2 ± 0.1 %ID in the control nodes (P < 0.0001). Conclusion: Biotin-liposomes encapsulating blue dye can be successfully labeled with 99mTc, providing a convenient option for the visualization and radiolocalization of the sentinel node. This biotin-liposome/avidin system may also have potential for the delivery of therapeutic drugs and radiopharmaceuticals to lymph nodes.

KW - TC

KW - Avidin

KW - Blue dye

KW - Liposomes

KW - Sentinel node

UR - http://www.scopus.com/inward/record.url?scp=0035090190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035090190&partnerID=8YFLogxK

M3 - Article

C2 - 11337521

AN - SCOPUS:0035090190

VL - 42

SP - 446

EP - 451

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

SN - 0161-5505

IS - 3

ER -