Use of resequencing oligonucleotide microarrays for identification of Streptococcus pyogenes and associated antibiotic resistance determinants

Louis Davignon, Elizabeth A Walter, Kate M. Mueller, Christopher P. Barrozo, David A. Stenger, Baochuan Lin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Group A streptococci (GAS) are responsible for a wide variety of human infections associated with considerable morbidity and mortality. Ever since the first systematic effort by Lancefield to group Streptococcus species by M protein variants, the detection and characterization of Streptococcus by different methods have been an evolving process. The ideal assay for GAS identification not only would provide quick and accurate diagnostic results but also would reveal antibiotic resistance patterns and genotype information, aiding not only in treatment but in epidemiologic assessment as well. The oligonucleotide microarray is a promising new technology which could potentially address this need. In this study, we evaluated the usefulness of oligonucleotide resequencing microarrays for identifying GAS and its associated antibiotic resistance markers. We demonstrated an assay platform that combines the use of resequencing DNA microarrays with either random nucleic acid amplification or multiplex PCR for GAS detection. When detecting Streptococcus pyogenes from coded clinical samples, this approach demonstrated an excellent concordance with a more established culture method. To this end, we showed the potential of resequencing microarrays for efficient and accurate detection of GAS and its associated antibiotic resistance markers with the benefit of sequencing information from microarray analysis.

Original languageEnglish (US)
Pages (from-to)5690-5695
Number of pages6
JournalJournal of Clinical Microbiology
Volume43
Issue number11
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Streptococcus pyogenes
Microbial Drug Resistance
Streptococcus
Oligonucleotide Array Sequence Analysis
Multiplex Polymerase Chain Reaction
Microarray Analysis
Nucleic Acids
Genotype
Technology
Morbidity
Mortality
Infection
Proteins

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Use of resequencing oligonucleotide microarrays for identification of Streptococcus pyogenes and associated antibiotic resistance determinants. / Davignon, Louis; Walter, Elizabeth A; Mueller, Kate M.; Barrozo, Christopher P.; Stenger, David A.; Lin, Baochuan.

In: Journal of Clinical Microbiology, Vol. 43, No. 11, 11.2005, p. 5690-5695.

Research output: Contribution to journalArticle

Davignon, Louis ; Walter, Elizabeth A ; Mueller, Kate M. ; Barrozo, Christopher P. ; Stenger, David A. ; Lin, Baochuan. / Use of resequencing oligonucleotide microarrays for identification of Streptococcus pyogenes and associated antibiotic resistance determinants. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 11. pp. 5690-5695.
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