Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit

Guadalupe Miranda, Cindy Kelly, Fortino Solorzano, Blanca Leanos, Rafael Coria, Jan E Patterson

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Serratia marcescens is a well-known cause of nosocomial infections and outbreaks, particularly in critically ill neonates and immunocompromised patients. Numerous methods have been proposed for typing. We used pulsed- held gel electrophoresis (PFGE) typing to analyze an outbreak in a neonatal intensive care unit (NICU). We included 23 patient isolates from an outbreak (March to July 1995), and 10 patient isolates from different wards during the same time period. PFGE of whole-cell DNA digested by SpeI was used as a marker of strain identity. The most common presentation of the infection was sepsis in 18 of 23 (78%) neonates. Only four different biotypes were identified; biotype A8d accounted for 84% of the strains. PFGE typing revealed two clones responsible for two different clonal strain dissemination outbreaks from March to July, with 24 patient isolates being pattern A and 4 patient isolates being pattern E. PFGE typing suggests cross transmission between patients in the NICU and other wards. The isolates from 5 other patients showed distinct PFGE patterns. Extensive investigation and cultures failed to identify any environmental or staff reservoir of S. marcescens. This is one of the first reports applying PFGE to the study of S. marcescens, and this method was a useful marker of strain identity. PFGE typing distinguished strains which appeared to be the same by biotyping.

Original languageEnglish (US)
Pages (from-to)3138-3141
Number of pages4
JournalJournal of Clinical Microbiology
Volume34
Issue number12
StatePublished - Dec 1996

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Serratia marcescens
Pulsed Field Gel Electrophoresis
Neonatal Intensive Care Units
Disease Outbreaks
Infection
Newborn Infant
Immunocompromised Host
Cross Infection
Critical Illness
Electrophoresis
Sepsis
Clone Cells
Gels
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit. / Miranda, Guadalupe; Kelly, Cindy; Solorzano, Fortino; Leanos, Blanca; Coria, Rafael; Patterson, Jan E.

In: Journal of Clinical Microbiology, Vol. 34, No. 12, 12.1996, p. 3138-3141.

Research output: Contribution to journalArticle

Miranda, Guadalupe ; Kelly, Cindy ; Solorzano, Fortino ; Leanos, Blanca ; Coria, Rafael ; Patterson, Jan E. / Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 12. pp. 3138-3141.
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