Use of oxygen-permeable silicone rubber pouches for growing mass cultures of bacteria

T. A. Giambernardi, R. J. Klebe

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Growth of most bacteria often involves the use of expensive incubated shaker systems. In this report, oxygen-permeable silicone rubber pouches, with oxygen permeability over 100 times higher than other polymers, were employed for growing bacterial cultures. With little, if any, agitation oxygen-permeable silicone rubber pouches produced bacterial growth rates equivalent to growth rates obtained in shaker flasks. The silicone rubber pouch described has a glass cuvette integrated into its design that permits readings of bacterial density without opening the pouch. One can sterilize and store powdered bacterial culture medium in silicone rubber pouches; therefore, bacterial cultures can be initiated by simply adding water and bacteria.

Original languageEnglish (US)
Pages (from-to)207-210
Number of pages4
JournalLetters in Applied Microbiology
Volume24
Issue number3
DOIs
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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