Use of near-infrared luminescent gold nanoclusters for detection of macrophages

Veronika Sapozhnikova, Brian Willsey, Reto Asmis, Tianyi Wang, James Travis Jenkins, Jacob Mancuso, Li Leo Ma, Roman Kuranov, Thomas E. Milner, Keith Johnston, Marc D Feldman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We determined the effect of aggregation and coating thickness of gold on the luminescence of nanoparticles engulfed by macrophages and in gelatin phantoms. Thin gold-coated iron oxide nanoclusters (nanoroses) have been developed to target macrophages to provide contrast enhancement for near-infrared optical imaging applications. We compare the brightness of nanoroses luminescent emissions in response to 635 nm laser excitation to other nanoparticles including nanoshells, nanorods, and Cy5 conjugated iron oxide nanoparticles. Luminescent properties of all these nanoparticles were investigated in monomeric and aggregated form in gelatin phantoms and primary macrophage cell cultures using confocal microscopy. Aggregation of the gold nanoparticles increased luminescence emission and correlated with increased surface mass of gold per nanoparticle (nanoshells 37 ± 14.30 × 10?3 brightness with 1.23 × 10?4 wt of gold (g)/nanoparticle versus original nanorose 1.45 ± 0.37 × 10?3 with 2.10 × 10?16 wt of gold/nanoparticle, p < 0.05). Nanoshells showed greater luminescent intensity than original nanoroses or Cy5 conjugated iron oxide nanoparticles when compared as nanoparticles per macrophage (38 ± 10 versus 11 ± 2.8 versus 17 ± 6.5, p < 0.05, respectively, ANOVA), but showed relatively poor macrophage uptake (1025 ± 128 versus 7549 ± 236 versus 96; 000 nanoparticles?cell, p < 0.05, student t-test nanoshells versus nanoroses). Enhancement of gold fluorescent emissions by nanoparticles can be achieved by reducing the thickness of the gold coating, by clustering the gold on the surface of the nanoparticles (nanoshells), and by clustering the gold nanoparticles themselves.

Original languageEnglish (US)
Article number026006
JournalJournal of Biomedical Optics
Volume17
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

macrophages
Macrophages
Nanoclusters
nanoclusters
Gold
gold
Nanoparticles
Infrared radiation
nanoparticles
Nanoshells
Iron oxides
iron oxides
gelatins
Gelatin
Luminescence
Luminance
brightness
Agglomeration
Gold coatings
luminescence

Keywords

  • Biophotonics
  • Imaging
  • Luminescence spectroscopy
  • Nanoparticles

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Use of near-infrared luminescent gold nanoclusters for detection of macrophages. / Sapozhnikova, Veronika; Willsey, Brian; Asmis, Reto; Wang, Tianyi; Jenkins, James Travis; Mancuso, Jacob; Ma, Li Leo; Kuranov, Roman; Milner, Thomas E.; Johnston, Keith; Feldman, Marc D.

In: Journal of Biomedical Optics, Vol. 17, No. 2, 026006, 02.2012.

Research output: Contribution to journalArticle

Sapozhnikova, V, Willsey, B, Asmis, R, Wang, T, Jenkins, JT, Mancuso, J, Ma, LL, Kuranov, R, Milner, TE, Johnston, K & Feldman, MD 2012, 'Use of near-infrared luminescent gold nanoclusters for detection of macrophages', Journal of Biomedical Optics, vol. 17, no. 2, 026006. https://doi.org/10.1117/1.JBO.17.2.026006
Sapozhnikova V, Willsey B, Asmis R, Wang T, Jenkins JT, Mancuso J et al. Use of near-infrared luminescent gold nanoclusters for detection of macrophages. Journal of Biomedical Optics. 2012 Feb;17(2). 026006. https://doi.org/10.1117/1.JBO.17.2.026006
Sapozhnikova, Veronika ; Willsey, Brian ; Asmis, Reto ; Wang, Tianyi ; Jenkins, James Travis ; Mancuso, Jacob ; Ma, Li Leo ; Kuranov, Roman ; Milner, Thomas E. ; Johnston, Keith ; Feldman, Marc D. / Use of near-infrared luminescent gold nanoclusters for detection of macrophages. In: Journal of Biomedical Optics. 2012 ; Vol. 17, No. 2.
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