Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium

Thomas Randall; T. Rajeswara Rao; C. Adinarayana Reddy

doi:10.1016/0006-291X(89)92659-4

Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium

Thomas Randall
, T. Rajeswara Rao
, C. Adinarayana Reddy

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A novel shuttle vector based spheroplast transformation system for the lignin degrading filamentous fungus P. chrysosporium is described. The transformation vector, designated pRR12, consists of the yeast integration plasmid YIp5, a putative autonomous replication sequence (ars) of P. chrysosporium, and a 2.2 kb PvuII fragment carrying kan^r determinant from plasmid pNG35, which confers resistance against both kanamycin and the related antibiotic G418. Two different strains of P. chrysosporium (ME446 and BKM-F) were transformed to G418 resistance using vector pRR12. Approximately 20 transformants per μg of vector DNA were obtained. The transforming vector pRR12 could be recovered from the total DNA of transformants by E. coli transformation, albeit at a low frequency.

Original language	English (US)
Pages (from-to)	720-725
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	161
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(89)92659-4
State	Published - Jun 15 1989
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/0006-291X(89)92659-4

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title = "Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium",

abstract = "A novel shuttle vector based spheroplast transformation system for the lignin degrading filamentous fungus P. chrysosporium is described. The transformation vector, designated pRR12, consists of the yeast integration plasmid YIp5, a putative autonomous replication sequence (ars) of P. chrysosporium, and a 2.2 kb PvuII fragment carrying kanr determinant from plasmid pNG35, which confers resistance against both kanamycin and the related antibiotic G418. Two different strains of P. chrysosporium (ME446 and BKM-F) were transformed to G418 resistance using vector pRR12. Approximately 20 transformants per μg of vector DNA were obtained. The transforming vector pRR12 could be recovered from the total DNA of transformants by E. coli transformation, albeit at a low frequency.",

author = "Thomas Randall and Rao, \{T. Rajeswara\} and Reddy, \{C. Adinarayana\}",

note = "Funding Information: by grant DE-FG02-85ER13369 from the Basic Biological Sciences; NSF grant We thank Novo Laboratories for",

year = "1989",

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doi = "10.1016/0006-291X(89)92659-4",

language = "English (US)",

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pages = "720--725",

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T1 - Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium

AU - Randall, Thomas

AU - Rao, T. Rajeswara

AU - Reddy, C. Adinarayana

N1 - Funding Information: by grant DE-FG02-85ER13369 from the Basic Biological Sciences; NSF grant We thank Novo Laboratories for

PY - 1989/6/15

Y1 - 1989/6/15

N2 - A novel shuttle vector based spheroplast transformation system for the lignin degrading filamentous fungus P. chrysosporium is described. The transformation vector, designated pRR12, consists of the yeast integration plasmid YIp5, a putative autonomous replication sequence (ars) of P. chrysosporium, and a 2.2 kb PvuII fragment carrying kanr determinant from plasmid pNG35, which confers resistance against both kanamycin and the related antibiotic G418. Two different strains of P. chrysosporium (ME446 and BKM-F) were transformed to G418 resistance using vector pRR12. Approximately 20 transformants per μg of vector DNA were obtained. The transforming vector pRR12 could be recovered from the total DNA of transformants by E. coli transformation, albeit at a low frequency.

AB - A novel shuttle vector based spheroplast transformation system for the lignin degrading filamentous fungus P. chrysosporium is described. The transformation vector, designated pRR12, consists of the yeast integration plasmid YIp5, a putative autonomous replication sequence (ars) of P. chrysosporium, and a 2.2 kb PvuII fragment carrying kanr determinant from plasmid pNG35, which confers resistance against both kanamycin and the related antibiotic G418. Two different strains of P. chrysosporium (ME446 and BKM-F) were transformed to G418 resistance using vector pRR12. Approximately 20 transformants per μg of vector DNA were obtained. The transforming vector pRR12 could be recovered from the total DNA of transformants by E. coli transformation, albeit at a low frequency.

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DO - 10.1016/0006-291X(89)92659-4

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SN - 0006-291X

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JF - Biochemical and Biophysical Research Communications

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ER -

Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium

Abstract

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