Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium

Thomas Randall, T. Rajeswara Rao, C. Adinarayana Reddy

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

A novel shuttle vector based spheroplast transformation system for the lignin degrading filamentous fungus P. chrysosporium is described. The transformation vector, designated pRR12, consists of the yeast integration plasmid YIp5, a putative autonomous replication sequence (ars) of P. chrysosporium, and a 2.2 kb PvuII fragment carrying kanr determinant from plasmid pNG35, which confers resistance against both kanamycin and the related antibiotic G418. Two different strains of P. chrysosporium (ME446 and BKM-F) were transformed to G418 resistance using vector pRR12. Approximately 20 transformants per μg of vector DNA were obtained. The transforming vector pRR12 could be recovered from the total DNA of transformants by E. coli transformation, albeit at a low frequency.

Original languageEnglish (US)
Pages (from-to)720-725
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number2
DOIs
StatePublished - Jun 15 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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