Usa1 protein facilitates substrate ubiquitylation through two separate domains

Ikjin Kim, Yue Li, Paulina Muniz, Hai Rao

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Background: Defects in protein folding are recognized as the root of many neurodegenerative disorders. In the endoplasmic reticulum (ER), secretory proteins are subjected to a stringent quality control process to eliminate misfolded proteins by the ER-associated degradation (ERAD) pathway. A novel ERAD component Usa1 was recently identified. However, the specific role of Usa1 in ERAD remains obscure. Methodology/Principal Findings: Here, we demonstrate that Usa1 is important for substrate ubiquitylation. Furthermore, we defined key cis-elements of Usa1 essential for its degradation function. Interestingly, a putative proteasome-binding motif is dispensable for the functioning of Usa1 in ERAD. We identify two separate cytosolic domains critical for Usa1 activity in ERAD, one of which is involved in binding to the Ub-protein ligase Hrd1/Hrd3. Usa1 may have another novel role in substrate ubiquitylation that is separate from the Hrd1 association. Conclusions/Significance: We conclude that Usa1 has two important roles in ERAD substrate ubiquitylation.

Original languageEnglish (US)
Article numbere7604
JournalPloS one
Volume4
Issue number10
DOIs
StatePublished - Oct 29 2009

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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