TY - JOUR
T1 - Urinary metabolomics fingerprinting around parturition identifies metabolites that differentiate lame dairy cows from healthy ones
AU - Eckel, E. F.
AU - Zhang, G.
AU - Dervishi, E.
AU - Zwierzchowski, G.
AU - Mandal, R.
AU - Wishart, D. S.
AU - Ametaj, B. N.
N1 - Funding Information:
We like to thank Genome Alberta (Calgary, Alberta, Canada), and Alberta Livestock and Meat Agency Ltd (Edmonton, Alberta, Canada) for funding this research project. We acknowledge the help of D. Hailemariam, S. A. Goldansaz, Q. Deng and J. F. Odhiambo in collection of samples from cows. We are also grateful to the technical staff at Dairy Research and Technology Center, University of Alberta, for their help and care with the cows. G. Zwierzchowski 0000-0003-1051-7591, The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript or in the decision to publish the results. This study was part of a prospective project designed to identify predictive biomarkers of periparturient diseases in dairy cows. All experimental procedures were approved by the University of Alberta Animal Policy and Welfare Committee for Livestock, and animals were cared for in accordance with the guidelines of the Canadian Council on Animal Care (1993). Metabolomics analyses were performed at the Metabolomics Innovation Centre, University of Alberta, Edmonton, AB, Canada. None of the data were deposited in an official repository.
Funding Information:
We like to thank Genome Alberta (Calgary, Alberta, Canada), and Alberta Livestock and Meat Agency Ltd (Edmonton, Alberta, Canada) for funding this research project. We acknowledge the help of D. Hailemariam, S. A. Goldansaz, Q. Deng and J. F. Odhiambo in collection of samples from cows. We are also grateful to the technical staff at Dairy Research and Technology Center, University of Alberta, for their help and care with the cows.
Publisher Copyright:
© 2020 The Animal Consortium
PY - 2020/1
Y1 - 2020/1
N2 - Lameness is a very important disorder of periparturient dairy cows with implications on milk production and composition as well as with consequences on reproductive performance. The aetiology of lameness is not clear although there have been various hypotheses suggested over the years. The objective of this study was to metabotype the urine of dairy cows prior to, during and after the onset of lameness by evaluating at weeks −8, −4 pre-calving, the week of lameness diagnosis, and +4 and +8 weeks post-calving. We used a metabolomics approach to analyse urine samples collected from dairy cows around calving (6 cows with lameness v. 20 healthy control cows). A total of 153 metabolites were identified and quantified using an in-house MS library and classified into 6 groups including: 11 amino acids (AAs), 39 acylcarnitines (ACs), 3 biogenic amines (BAs), 84 glycerophospholipids, 15 sphingolipids and hexose. A total of 23, 36, 40, 23 and 49 metabolites were observed to be significantly different between the lame and healthy cows at −8 and −4 weeks pre-calving, week of lameness diagnosis as well as at +4 and +8 weeks post-calving, respectively. It should be noted that most of the identified metabolites were elevated; however, a few of them were also lower in lame cows. Overall, ACs and glycerophospholipids, specifically phosphatidylcholines (PCs), were the metabolite groups displaying the strongest differences in the urine of pre-lame and lame cows. Lysophosphatidylcholines (LysoPCs), although to a lesser extent than PCs, were altered at all time points. Alterations in urinary AA concentrations were also observed during the current study for four time points. During the pre-calving period, there was an observed elevation of arginine (−8 week), tyrosine (−8 week) and aspartate (−4 week), as well as a depression of urinary glutamate (−4 weeks). In the current study, it was additionally observed that concentrations of several sphingomyelins and one BA were altered in pre-lame and lame cows. Symmetric dimethylarginine was elevated at both −8 weeks pre-calving and the week of lameness diagnosis. Data showed that urinary fingerprinting might be a reliable methodology to be used in the future to differentiate lame cows from healthy ones.
AB - Lameness is a very important disorder of periparturient dairy cows with implications on milk production and composition as well as with consequences on reproductive performance. The aetiology of lameness is not clear although there have been various hypotheses suggested over the years. The objective of this study was to metabotype the urine of dairy cows prior to, during and after the onset of lameness by evaluating at weeks −8, −4 pre-calving, the week of lameness diagnosis, and +4 and +8 weeks post-calving. We used a metabolomics approach to analyse urine samples collected from dairy cows around calving (6 cows with lameness v. 20 healthy control cows). A total of 153 metabolites were identified and quantified using an in-house MS library and classified into 6 groups including: 11 amino acids (AAs), 39 acylcarnitines (ACs), 3 biogenic amines (BAs), 84 glycerophospholipids, 15 sphingolipids and hexose. A total of 23, 36, 40, 23 and 49 metabolites were observed to be significantly different between the lame and healthy cows at −8 and −4 weeks pre-calving, week of lameness diagnosis as well as at +4 and +8 weeks post-calving, respectively. It should be noted that most of the identified metabolites were elevated; however, a few of them were also lower in lame cows. Overall, ACs and glycerophospholipids, specifically phosphatidylcholines (PCs), were the metabolite groups displaying the strongest differences in the urine of pre-lame and lame cows. Lysophosphatidylcholines (LysoPCs), although to a lesser extent than PCs, were altered at all time points. Alterations in urinary AA concentrations were also observed during the current study for four time points. During the pre-calving period, there was an observed elevation of arginine (−8 week), tyrosine (−8 week) and aspartate (−4 week), as well as a depression of urinary glutamate (−4 weeks). In the current study, it was additionally observed that concentrations of several sphingomyelins and one BA were altered in pre-lame and lame cows. Symmetric dimethylarginine was elevated at both −8 weeks pre-calving and the week of lameness diagnosis. Data showed that urinary fingerprinting might be a reliable methodology to be used in the future to differentiate lame cows from healthy ones.
KW - biomarkers
KW - lameness
KW - metabolomics
KW - transition dairy cows
KW - urine
UR - http://www.scopus.com/inward/record.url?scp=85087176025&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087176025&partnerID=8YFLogxK
U2 - 10.1017/S1751731120001172
DO - 10.1017/S1751731120001172
M3 - Article
C2 - 32498732
AN - SCOPUS:85087176025
SN - 1751-7311
VL - 14
SP - 2138
EP - 2149
JO - Animal
JF - Animal
IS - 10
ER -