Unraveling the mechanistic features of RNA polymerase II termination by the 5'-3' exoribonuclease Rat1

Jieun Park, Myungjin Kang, Minkyu Kim

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Within a complex with Rai1, the 5'-3' exoribonuclease Rat1 promotes termination of RNA polymerase II (RNAPII) on protein-coding genes, but its underlying molecular mechanism is still poorly understood. Using in vitro transcription termination assays, we have found that RNAPII is prone to more effective termination by Rat1/Rai1 when its catalytic site is disrupted due to NTP misincorporation, implying that paused RNAPII, which is often found in vivo near termination sites, could adopt a similar configuration to Rat1/Rai1 and trigger termination. Intriguingly, yeast Rat1/Rai1 does not terminate Escherichia coli RNAP, implying that a specific interaction between Rat1/Rai1 and RNAPII may be required for termination. Furthermore, the efficiency of termination increases as the RNA transcript undergoing degradation by Rat1 gets longer, which suggests that Rat1 may generate a driving force for dissociating RNAPII from the template while degrading the nascent transcripts to catch up to the polymerase. These results indicate thatmultiple mechanistic features contribute to Rat1-mediated termination of RNAPII.

Original languageEnglish (US)
Pages (from-to)2625-2637
Number of pages13
JournalNucleic acids research
Volume43
Issue number5
DOIs
StatePublished - Mar 11 2015
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'Unraveling the mechanistic features of RNA polymerase II termination by the 5'-3' exoribonuclease Rat1'. Together they form a unique fingerprint.

Cite this