Understanding the bone/implant interface

B. D. Boyan, V. L. Sylvia, D. D. Dean, C. H. Lohmann, D. L. Cochran, Z. Schwartz

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations


In order to better understand how implant surfaces modulate bone formation, we developed a cell culture model. Cells in the osteoblastic lineage (fetal rat calvarial cells [FRCs]: multipotent osteoprogenitor cells; human osteoblast-like MG63 cells: immature osteoblasts; mouse OCT-1 cells: mature osteoblasts; and mouse MLO-Y4 cells: osteocyte-like cells) were cultured on cpTi disks with either smooth (R(a) = 0.5 μm) or rough (R(a) = 5 μm) surfaces. The effects of surface roughness on proliferation, matrix synthesis, differentiation, local factor production, cell morphology, and response to 1,25-(OH)2D3 were determined. The interaction of surface roughness and surface chemistry in cell response was also examined by culturing MG63 cells on smooth and rough surfaces prepared by machining disks of cpTi or Ti-6Al-4V alloy (Ti-A). The results show that cell differentiation and local factor production are enhanced with increasing surface roughness, whereas proliferation is decreased. Response to 1,25-(OH)2D3 increases with increasing surface roughness. Both surface roughness and 1,25-(OH)2D3 mediate their effects through PGE2, explaining, in part, the synergistic effects of these two variables. While surface chemistry plays a role in determining cell response, surface roughness may be a more important variable with respect to bone formation.

Original languageEnglish (US)
Pages (from-to)123-133
Number of pages11
Issue number3
StatePublished - 1999


  • 1,25-(OH)D
  • Alkaline phosphatase
  • Bone formation
  • Osteoblasts
  • Osteocalcin
  • PGE
  • Proliferation
  • Surface roughness
  • TGF- β1
  • Titanium

ASJC Scopus subject areas

  • General Medicine


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