Ultraviolet radiation-induced DNA damage in promoter elements inhibits gene expression

Rita Ghosh, Ramakumar Tummala, David L. Mitchell

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Repair of DNA damage in gene promoters is slower than in actively transcribed genes. Persistent damage in gene promoters though transient can have significant biological effects on regulated gene expression. In this study we investigated the effect of ultraviolet radiation on gene promoter-associated functions when DNA damage is located within and outside transcription factor binding sites. Our results show that both cyclobutane pyrimidine dimers and (6-4) photoproducts inhibit DNA-protein interaction, in vitro transcript production and transactivation of reporter genes. The biological significance of transient DNA damage as a mechanism in carcinogenesis is discussed.

Original languageEnglish (US)
Pages (from-to)427-432
Number of pages6
JournalFEBS Letters
Volume554
Issue number3
DOIs
StatePublished - Nov 20 2003
Externally publishedYes

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Keywords

  • DNA damage
  • Gene expression
  • Ultraviolet radiation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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