Ultrastructure of transganglionic HRP transport in cat trigeminal system

Michael A. Henry, Lesnick E. Westrum, Lonnie R. Johnson

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The ultrastructure of transganglionic transport of horseradish peroxidase (HRP) from the inferior alveolar (IA) nerve to the brainstem is being studied in the cat. The IA nerve was soaked in an HRP solution and following a two-day survival the animal was perfused transcardially with a paraformaldehyde-glutaraldehyde solution. The tissue was immediately dissected and postfixed for 1-3 h in perfusate. Sections of 75 μm thickness were cut with a Vibratome and reacted utilizing tetramethyl benzidine (TMB) as the chromagen. Optimum results for electron microscopy were obtained by osmication in a pH 6.0, 1% osmium tetroxide solution for 45 min at 45 °C, followed by rapid dehydration and embedment in Epon. The resulting HRP-TMB reaction product was characterized and identified ultrastructurally in ganglion cells, peripheral and central axons and in brainstem terminals. The HRP-TMB reaction product varied in density but had consistent crystalline-like laminations of a repeating unit and characterized by a membrane 4-5 nm in diameter. Some of the HRP-TMB reaction product found in terminals and axons was below the limit of resolution of the light microscope.

Original languageEnglish (US)
Pages (from-to)255-266
Number of pages12
JournalBrain Research
Volume334
Issue number2
DOIs
StatePublished - May 20 1985

Keywords

  • electron microscopy
  • horseradish peroxidase
  • transganglionic transport
  • trigeminal

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Ultrastructure of transganglionic HRP transport in cat trigeminal system'. Together they form a unique fingerprint.

Cite this