Ultrasound-Targeted Microbubble Destruction Mediates Gene Transfection for Beta-Cell Regeneration and Glucose Regulation

Chao Zhang; Shuyuan Chen; Qunying Li; Jiao Wu; Fuqiang Qiu; Zhiyi Chen; Yang Sun; Jieli Luo; Raul A. Bastarrachea; Paul A. Grayburn; Ralph A. DeFronzo; Yajing Liu; Kun Qian; Pintong Huang

doi:10.1002/smll.202008177

Ultrasound-Targeted Microbubble Destruction Mediates Gene Transfection for Beta-Cell Regeneration and Glucose Regulation

Chao Zhang, Shuyuan Chen, Qunying Li, Jiao Wu, Fuqiang Qiu, Zhiyi Chen, Yang Sun, Jieli Luo, Raul A. Bastarrachea, Paul A. Grayburn, Ralph A. DeFronzo, Yajing Liu, Kun Qian, Pintong Huang

Division of Diabetes

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Ultrasound-targeted microbubble destruction (UTMD) mediates gene transfection with high biosafety and thus has been promising toward treatment of type 1 diabetes. However, the potential application of UTMD in type 2 diabetes (T2D) is still limited, due to the lack of systematic design and dynamic monitoring. Herein, an efficient gene delivery system is constructed by plasmid deoxyribonucleic acid (DNA) encoding glucagon-like peptide 1 (GLP-1) in ultrasound-induced microbubbles, toward treatment of T2D in macaque. The as designed UTMD afforded enhancement of cell membrane penetration and GLP-1 expression in macaque, which is characterized by ultrasound-guided biopsy to monitor the dynamic process of islet cells for 6 months. Also, improvement of pancreatic beta cell regeneration, and regulation of plasma glucose in macaque with T2D is achieved. The approach would serve as promising alternatives for the treatment of T2D.

Original language	English (US)
Article number	2008177
Journal	Small
Volume	17
Issue number	31
DOIs	https://doi.org/10.1002/smll.202008177
State	Published - Aug 5 2021

Keywords

cell metabolism
diabetes
gene transfection
microbubbles
ultrasound

ASJC Scopus subject areas

Biotechnology
General Chemistry
Biomaterials
General Materials Science
Engineering (miscellaneous)

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10.1002/smll.202008177

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title = "Ultrasound-Targeted Microbubble Destruction Mediates Gene Transfection for Beta-Cell Regeneration and Glucose Regulation",

abstract = "Ultrasound-targeted microbubble destruction (UTMD) mediates gene transfection with high biosafety and thus has been promising toward treatment of type 1 diabetes. However, the potential application of UTMD in type 2 diabetes (T2D) is still limited, due to the lack of systematic design and dynamic monitoring. Herein, an efficient gene delivery system is constructed by plasmid deoxyribonucleic acid (DNA) encoding glucagon-like peptide 1 (GLP-1) in ultrasound-induced microbubbles, toward treatment of T2D in macaque. The as designed UTMD afforded enhancement of cell membrane penetration and GLP-1 expression in macaque, which is characterized by ultrasound-guided biopsy to monitor the dynamic process of islet cells for 6 months. Also, improvement of pancreatic beta cell regeneration, and regulation of plasma glucose in macaque with T2D is achieved. The approach would serve as promising alternatives for the treatment of T2D.",

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author = "Chao Zhang and Shuyuan Chen and Qunying Li and Jiao Wu and Fuqiang Qiu and Zhiyi Chen and Yang Sun and Jieli Luo and Bastarrachea, {Raul A.} and Grayburn, {Paul A.} and DeFronzo, {Ralph A.} and Yajing Liu and Kun Qian and Pintong Huang",

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doi = "10.1002/smll.202008177",

language = "English (US)",

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AU - Zhang, Chao

AU - Chen, Shuyuan

AU - Li, Qunying

AU - Wu, Jiao

AU - Qiu, Fuqiang

AU - Chen, Zhiyi

AU - Sun, Yang

AU - Luo, Jieli

AU - Bastarrachea, Raul A.

AU - Grayburn, Paul A.

AU - DeFronzo, Ralph A.

AU - Liu, Yajing

AU - Qian, Kun

AU - Huang, Pintong

PY - 2021/8/5

Y1 - 2021/8/5

N2 - Ultrasound-targeted microbubble destruction (UTMD) mediates gene transfection with high biosafety and thus has been promising toward treatment of type 1 diabetes. However, the potential application of UTMD in type 2 diabetes (T2D) is still limited, due to the lack of systematic design and dynamic monitoring. Herein, an efficient gene delivery system is constructed by plasmid deoxyribonucleic acid (DNA) encoding glucagon-like peptide 1 (GLP-1) in ultrasound-induced microbubbles, toward treatment of T2D in macaque. The as designed UTMD afforded enhancement of cell membrane penetration and GLP-1 expression in macaque, which is characterized by ultrasound-guided biopsy to monitor the dynamic process of islet cells for 6 months. Also, improvement of pancreatic beta cell regeneration, and regulation of plasma glucose in macaque with T2D is achieved. The approach would serve as promising alternatives for the treatment of T2D.

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Ultrasound-Targeted Microbubble Destruction Mediates Gene Transfection for Beta-Cell Regeneration and Glucose Regulation

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Keywords

ASJC Scopus subject areas

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