Type II iodothyronine 5'-deiodinase in mouse brown adipose tissue is stimulated by a single injection of isoproterenol in an oil/water emulsion

A. Lerchl, K. A. Stokkan, K. O. Nonaka, M. K. Vaughan, Russel J Reiter

Research output: Contribution to journalArticle

Abstract

A single subcutaneous morning injection of the β-adrenergic agonist isoproterenol (ISO) (10 mg/kg body weight) in an oil/water emulsion (70/30; v/v) caused a marked increase in the activity of the enzyme type II iodothyronine 5'-deiodinase (5'-D II) in the interscapular brown adipose tissue of BDF-1 mice. After a delay of 4 hours, the 5'-D II activity began to rise in an almost linear fashion and was increased 3-fold after 8 hours, when compared to the control values. The results indicate that this method of ISO administration may be a valid tool for artificial stimulation of brown adipose tissue in animals by β-adrenergic agonists.

Original languageEnglish (US)
Pages (from-to)197-199
Number of pages3
JournalExperimental and Clinical Endocrinology
Volume101
Issue number3
StatePublished - 1993
Externally publishedYes

Fingerprint

Iodide Peroxidase
Adrenergic Agonists
Brown Adipose Tissue
Emulsions
Isoproterenol
Oils
Injections
Water
Subcutaneous Injections
Body Weight
Enzymes
iodothyronine deiodinase type II

Keywords

  • β-adrenergic receptor
  • Brown fat
  • Isoproterenol
  • Type II iodothyronine 5'-deiodinase

ASJC Scopus subject areas

  • Endocrinology

Cite this

Type II iodothyronine 5'-deiodinase in mouse brown adipose tissue is stimulated by a single injection of isoproterenol in an oil/water emulsion. / Lerchl, A.; Stokkan, K. A.; Nonaka, K. O.; Vaughan, M. K.; Reiter, Russel J.

In: Experimental and Clinical Endocrinology, Vol. 101, No. 3, 1993, p. 197-199.

Research output: Contribution to journalArticle

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AU - Reiter, Russel J

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