Type II iodothyronine 5'-deiodinase in mouse brown adipose tissue is stimulated by a single injection of isoproterenol in an oil/water emulsion

A. Lerchl, K. A. Stokkan, K. O. Nonaka, M. K. Vaughan, R. J. Reiter

Research output: Contribution to journalArticle

Abstract

A single subcutaneous morning injection of the β-adrenergic agonist isoproterenol (ISO) (10 mg/kg body weight) in an oil/water emulsion (70/30; v/v) caused a marked increase in the activity of the enzyme type II iodothyronine 5'-deiodinase (5'-D II) in the interscapular brown adipose tissue of BDF-1 mice. After a delay of 4 hours, the 5'-D II activity began to rise in an almost linear fashion and was increased 3-fold after 8 hours, when compared to the control values. The results indicate that this method of ISO administration may be a valid tool for artificial stimulation of brown adipose tissue in animals by β-adrenergic agonists.

Original languageEnglish (US)
Pages (from-to)197-199
Number of pages3
JournalExperimental and Clinical Endocrinology
Volume101
Issue number3
StatePublished - Jan 1 1993
Externally publishedYes

    Fingerprint

Keywords

  • Brown fat
  • Isoproterenol
  • Type II iodothyronine 5'-deiodinase
  • β-adrenergic receptor

ASJC Scopus subject areas

  • Endocrinology

Cite this