Two polymorphic microsatellites in a coding segment of the canine androgen receptor gene.

H. Shibuya, D. J. Nonneman, T. H. Huang, V. K. Ganjam, F. A. Mann, Hui-ming Huang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A 0.6-kb segment of exon 1 of the canine androgen receptor gene contains two polymorphic CAG tandem repeats which encode strings of glutamine homopolymers. The number of CAGs in each tandem repeat was determined by (1) polymerase chain reaction (PCR) amplification of a gene segment containing both repeats, (2) cleavage between repeats with restriction enzyme EcoO109I and (3) fractionation of the restriction fragments containing individual CAG repeats by denaturing polyacrylamide gel electrophoresis (PAGE). Individual genomic DNA samples from 80 unrelated dogs (53 males plus 27 females for a total of 107 X chromosomes) contained 10-12 CAGs in the 5' repeats and 10-13 CAGs in the 3' repeats. Thirteen distinct androgen receptor genotypes were identified. Eleven (or 41%) of the 27 unrelated females were heterozygous in one or both repeat regions, whereas all male samples produced single bands as expected for X chromosome markers. A total of seven distinct haplotypes contributed to the 13 genotypes. The 'polymorphism information content' or 'PIC' for this seven-allele X chromosome marker was 0.67.

Original languageEnglish (US)
Pages (from-to)345-348
Number of pages4
JournalAnimal Genetics
Volume24
Issue number5
StatePublished - Oct 1993
Externally publishedYes

Fingerprint

Androgen Receptors
X Chromosome
X chromosome
Microsatellite Repeats
Canidae
Tandem Repeat Sequences
tandem repeat sequences
microsatellite repeats
Genetic Markers
dogs
polymerase chain reaction
Genotype
Genes
Chromosomes, Human, Pair 10
genes
Gene Amplification
genotype
Glutamine
glutamine
Haplotypes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

Shibuya, H., Nonneman, D. J., Huang, T. H., Ganjam, V. K., Mann, F. A., & Huang, H. (1993). Two polymorphic microsatellites in a coding segment of the canine androgen receptor gene. Animal Genetics, 24(5), 345-348.

Two polymorphic microsatellites in a coding segment of the canine androgen receptor gene. / Shibuya, H.; Nonneman, D. J.; Huang, T. H.; Ganjam, V. K.; Mann, F. A.; Huang, Hui-ming.

In: Animal Genetics, Vol. 24, No. 5, 10.1993, p. 345-348.

Research output: Contribution to journalArticle

Shibuya, H, Nonneman, DJ, Huang, TH, Ganjam, VK, Mann, FA & Huang, H 1993, 'Two polymorphic microsatellites in a coding segment of the canine androgen receptor gene.', Animal Genetics, vol. 24, no. 5, pp. 345-348.
Shibuya H, Nonneman DJ, Huang TH, Ganjam VK, Mann FA, Huang H. Two polymorphic microsatellites in a coding segment of the canine androgen receptor gene. Animal Genetics. 1993 Oct;24(5):345-348.
Shibuya, H. ; Nonneman, D. J. ; Huang, T. H. ; Ganjam, V. K. ; Mann, F. A. ; Huang, Hui-ming. / Two polymorphic microsatellites in a coding segment of the canine androgen receptor gene. In: Animal Genetics. 1993 ; Vol. 24, No. 5. pp. 345-348.
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