Two polymorphic microsatellites in a coding segment of the canine androgen receptor gene

H. Shibuya, D. J. Nonneman, V. K. Ganjam, F. A. Mann, G. S. Johnson, T. H‐M Huang

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A 0.6‐kb segment of exon 1 of the canine androgen receptor gene contains two polymorphic CAG tandem repeats which encode strings of glutamine homopolymers. The number of CAGs in each tandem repeat was determined by (1) polymerase chain reaction (PCR) amplification of a gene segment containing both repeats, (2) cleavage between repeats with restriction enzyme EcoO109I and (3) fractionation of the restriction fragments containing individual CAG repeats by denaturing polyacrylamide gel electrophoresis (PAGE). Individual genomic DNA samples from 80 unrelated dogs (53 males plus 27 females for a total of 107 X chromosomes) contained 10–12 CAGs in the 5′ repeats and 10–13 CAGs in the 3′ repeats. Thirteen distinct androgen receptor genotypes were identified. Eleven (or 41%) of the 27 unrelated females were heterozygous in one or both repeat regions, whereas all male samples produced single bands as expected for X chromosome markers. A total of seven distinct haplotypes contributed to the 13 genotypes. The ‘polymorphism information content’ or PIC for this seven‐allele X chromosome marker was 0.67.

Original languageEnglish (US)
Pages (from-to)345-348
Number of pages4
JournalAnimal Genetics
Volume24
Issue number5
DOIs
StatePublished - Oct 1993
Externally publishedYes

Keywords

  • X‐chromosome marker
  • androgen receptor gene
  • dog
  • micro‐satellite

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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