Two novel cSNPs of weaver gene in Chinese indigenous goat and their associations with milk yield

Zhuanjian Li, Zhongqi Chen, Xianyong Lan, Liang Ma, Yujiao Qu, Yanli Liu, Mijie Li, Ping Wang, Fei Li, Hong Chen

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Weaver gene plays an essential physiological role in the function of many organs, including brain, heart, kidney and endocrine cells, and also in the regulation of insulin secretion by glucose and/or neurotransmitters. Thus, weaver gene is an important potential candidate gene effecting on performance traits. The objective of this study was to detect the genetic variation of five loci within weaver gene by PCR-SSCP, DNA sequencing and forced PCR-RFLP methods in 1,019 Chinese indigenous goats. Two novel coding SNPs (XM-598993:m.864G>A; XM-598993:m.1224T>A) locating on P3 and P4 loci were identified and detected by MluI and AsuII forced PCR-RFLP, respectively. In the MluI analysis, the frequencies of goat MluI-A allele in the analyzed populations were 0.226, 0.248, 0.096 and 0.088 for XNSN, GZ, SBWC and XJWC, respectively. Genotypic frequencies were found to be significantly different in four breeds (χ2 = 75.842, df = 6, P < 0.001); In the AsuII analysis, the frequencies of goat AsuII-A allele in the analyzed populations were 0.584, 0.441, 0.073 and 0.063 for XNSN, GZ, SBWC and XJWC, respectively. Genotypic frequencies were found to be significantly different among four breeds (χ2 = 399.464, df = 6, P < 0.001). The frequencies of allele MluI-A and AsuII-A in XNSN and GZ populations were significantly higher than those of SBWC and XJWC goats. Association analysis with adjusted milk yield in the XNSN breed indicated that the animals with AsuII-AA genotype owned significantly higher adjusted milk yield than the ones with AsuII-TT genotype in the second lactation (P < 0.05). The observation suggested that the allele "AsuII-A" had the positive effects on adjusted milk yield in the second lactation.

Original languageEnglish (US)
Pages (from-to)563-569
Number of pages7
JournalMolecular Biology Reports
Volume37
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

Keywords

  • Forced PCR-RFLP
  • Genetic variation
  • Goat
  • Milk yield
  • Weaver gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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