Two nonallelic tRNA(i)(Met) genes are located in the p23→q12 region of human chromosome 6

S. L. Naylor; A. Y. Sakaguchi; T. B. Shows

doi:10.1073/pnas.80.16.5027

Two nonallelic tRNA(i)(Met) genes are located in the p23→q12 region of human chromosome 6

S. L. Naylor, A. Y. Sakaguchi, T. B. Shows

Research output: Contribution to journal › Article › peer-review

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Abstract

Two nonallelic human tRNA(i)(Met) genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each tRNA(i)(Met) gene. These unique sequence probes thus allowed each tRNA(i)(Met) gene to be analyzed individually in cell hybrids. Both tRna(i)(Met) genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into five segments, both tRNA(i)(Met) genes were assigned to the p23→q12 region. These results raise the possibility that other tRNA(i)(Met) genes may be syntenic with the two described in this study and illustrate the utility of using unique lanking sequences to identify members of a multigene family.

Original language	English (US)
Pages (from-to)	5027-5031
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	80
Issue number	16 I
DOIs	https://doi.org/10.1073/pnas.80.16.5027
State	Published - 1983
Externally published	Yes

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title = "Two nonallelic tRNA(i)(Met) genes are located in the p23→q12 region of human chromosome 6",

abstract = "Two nonallelic human tRNA(i)(Met) genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each tRNA(i)(Met) gene. These unique sequence probes thus allowed each tRNA(i)(Met) gene to be analyzed individually in cell hybrids. Both tRna(i)(Met) genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into five segments, both tRNA(i)(Met) genes were assigned to the p23→q12 region. These results raise the possibility that other tRNA(i)(Met) genes may be syntenic with the two described in this study and illustrate the utility of using unique lanking sequences to identify members of a multigene family.",

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year = "1983",

doi = "10.1073/pnas.80.16.5027",

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T1 - Two nonallelic tRNA(i)(Met) genes are located in the p23→q12 region of human chromosome 6

AU - Naylor, S. L.

AU - Sakaguchi, A. Y.

AU - Shows, T. B.

PY - 1983

Y1 - 1983

N2 - Two nonallelic human tRNA(i)(Met) genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each tRNA(i)(Met) gene. These unique sequence probes thus allowed each tRNA(i)(Met) gene to be analyzed individually in cell hybrids. Both tRna(i)(Met) genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into five segments, both tRNA(i)(Met) genes were assigned to the p23→q12 region. These results raise the possibility that other tRNA(i)(Met) genes may be syntenic with the two described in this study and illustrate the utility of using unique lanking sequences to identify members of a multigene family.

AB - Two nonallelic human tRNA(i)(Met) genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each tRNA(i)(Met) gene. These unique sequence probes thus allowed each tRNA(i)(Met) gene to be analyzed individually in cell hybrids. Both tRna(i)(Met) genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into five segments, both tRNA(i)(Met) genes were assigned to the p23→q12 region. These results raise the possibility that other tRNA(i)(Met) genes may be syntenic with the two described in this study and illustrate the utility of using unique lanking sequences to identify members of a multigene family.

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Two nonallelic tRNA(i)(Met) genes are located in the p23→q12 region of human chromosome 6

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