Two-dimensional spectroscopy of electrophoretic gels

Robert J. Klebe, Melodee G. Mancuso, Carol R. Brown, Lilly Teng

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Optical techniques are described which permit one to analyze two-dimensional electrophoretic gels in a fashion which is analogous to the one-dimensional spectroscopy of solutions. In the methods described, an electrophoretic gel is irradiated with monochromatic light and isozyme patterns are detected by the absorption of light or the fluorescent emission of light. The system described can both generate and detect monochromatic light in a range from 200 to 1100 nm. Without the use of histochemical stains, several isozymes have been visualized by purely optical means. Five methods for the visualization of lactate dehydrogenase and five methods for the demonstration of trypsin isozymes are described. In addition, general methods have been formulated for hydrolases and oxidases. Gel spectroscopy should permit the investigation of a wide range of new isozymes.

Original languageEnglish (US)
Pages (from-to)655-672
Number of pages18
JournalBiochemical Genetics
Issue number7-8
StatePublished - Aug 1981
Externally publishedYes


  • electrophoresis
  • isozymes
  • lactate dehydrogenace
  • somatic cell genetics
  • spectroscopy
  • trypsin

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics


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