Two-dimensional agarose gel electrophoresis without gel manipulation

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The apparatus and procedure to perform two-dimensional agarose gel electrophoresis without manipulating the gel used for the first electrophoresis (first-dimension gel) have been developed. The procedure is less complex, less damaging to first-dimension gels, and more precise than procedures that require manipulation of the first-dimension gel. When combined with gel-embedding techniques, the procedure presented can be used to perform the second electrophoresis in a gel different from the first-dimension gel. A first-dimension gel too dilute to be manipulated and a more concentrated gel for the second electrophoresis have been used to separate DNA open circles from a mixture of variable-length linear DNAs.

Original languageEnglish (US)
Pages (from-to)172-178
Number of pages7
JournalAnalytical Biochemistry
Volume144
Issue number1
DOIs
StatePublished - Jan 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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