Twenty-four hour variation of α1-adrenergic receptors in the pineal gland of the male Syrian hamster

Andreas Pangerl; Brigitte Pangerl; Russel J. Reiter; George M. Vaughan; David J. Jones

doi:10.1016/0006-8993(89)90445-9

Twenty-four hour variation of α₁-adrenergic receptors in the pineal gland of the male Syrian hamster

Andreas Pangerl, Brigitte Pangerl, Russel J. Reiter, George M. Vaughan, David J. Jones

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Specific binding of [¹²⁵I]iodo-[β-(4-hydroxyphenyl)-ethylaminomethyl]tetralo ne ([¹²⁵I]HEAT) was used to assess α₁-adrenergic receptors on pineal gland membranes of male Syrian hamsters (Mesocricetus auratus) housed under a 14:10 h light-dark cycle (lights on at 06.00 h). Saturation experiments with pooled pineal membrane preparations showed the presence of α₁-adrenergic receptor sites (dissociation constant K_d approx. 0.1 nM). Analysis of 4 time points indicated no significant change in K_d, but significant (P < 0.01) changes of receptor density (B_max) with a minimum recorded at night. Binding of a constant amount of [¹²⁵I]HEAT (200 pM) to pineal membranes at 8 time points exhibited a circadian variation (P < 0.001) of receptor density with lowest values around midnight and highest levels during daytime.

Original language	English (US)
Pages (from-to)	166-169
Number of pages	4
Journal	Brain Research
Volume	490
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(89)90445-9
State	Published - Jun 19 1989

Keywords

Circadian variation
Pineal gland
α-Adrenoceptor

ASJC Scopus subject areas

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

Access to Document

10.1016/0006-8993(89)90445-9

Cite this

@article{dbfdecc0e0e0434c8d4e6ba1f1516d21,

title = "Twenty-four hour variation of α1-adrenergic receptors in the pineal gland of the male Syrian hamster",

abstract = "Specific binding of [125I]iodo-[β-(4-hydroxyphenyl)-ethylaminomethyl]tetralo ne ([125I]HEAT) was used to assess α1-adrenergic receptors on pineal gland membranes of male Syrian hamsters (Mesocricetus auratus) housed under a 14:10 h light-dark cycle (lights on at 06.00 h). Saturation experiments with pooled pineal membrane preparations showed the presence of α1-adrenergic receptor sites (dissociation constant Kd approx. 0.1 nM). Analysis of 4 time points indicated no significant change in Kd, but significant (P < 0.01) changes of receptor density (Bmax) with a minimum recorded at night. Binding of a constant amount of [125I]HEAT (200 pM) to pineal membranes at 8 time points exhibited a circadian variation (P < 0.001) of receptor density with lowest values around midnight and highest levels during daytime.",

keywords = "Circadian variation, Pineal gland, α-Adrenoceptor",

author = "Andreas Pangerl and Brigitte Pangerl and Reiter, {Russel J.} and Vaughan, {George M.} and Jones, {David J.}",

note = "Funding Information: B.P. was supported by the Feodor Lynen program of the Alexander von Humboldt Foundation, Bonn, F.R.G. We thank M.K. Vaughan and G.R. Buzzell for assistance with collecting tissue. Research supported by NSF Grant DCB 87-11241.",

year = "1989",

month = jun,

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doi = "10.1016/0006-8993(89)90445-9",

language = "English (US)",

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AU - Pangerl, Andreas

AU - Pangerl, Brigitte

AU - Reiter, Russel J.

AU - Vaughan, George M.

AU - Jones, David J.

N1 - Funding Information: B.P. was supported by the Feodor Lynen program of the Alexander von Humboldt Foundation, Bonn, F.R.G. We thank M.K. Vaughan and G.R. Buzzell for assistance with collecting tissue. Research supported by NSF Grant DCB 87-11241.

PY - 1989/6/19

Y1 - 1989/6/19

N2 - Specific binding of [125I]iodo-[β-(4-hydroxyphenyl)-ethylaminomethyl]tetralo ne ([125I]HEAT) was used to assess α1-adrenergic receptors on pineal gland membranes of male Syrian hamsters (Mesocricetus auratus) housed under a 14:10 h light-dark cycle (lights on at 06.00 h). Saturation experiments with pooled pineal membrane preparations showed the presence of α1-adrenergic receptor sites (dissociation constant Kd approx. 0.1 nM). Analysis of 4 time points indicated no significant change in Kd, but significant (P < 0.01) changes of receptor density (Bmax) with a minimum recorded at night. Binding of a constant amount of [125I]HEAT (200 pM) to pineal membranes at 8 time points exhibited a circadian variation (P < 0.001) of receptor density with lowest values around midnight and highest levels during daytime.

AB - Specific binding of [125I]iodo-[β-(4-hydroxyphenyl)-ethylaminomethyl]tetralo ne ([125I]HEAT) was used to assess α1-adrenergic receptors on pineal gland membranes of male Syrian hamsters (Mesocricetus auratus) housed under a 14:10 h light-dark cycle (lights on at 06.00 h). Saturation experiments with pooled pineal membrane preparations showed the presence of α1-adrenergic receptor sites (dissociation constant Kd approx. 0.1 nM). Analysis of 4 time points indicated no significant change in Kd, but significant (P < 0.01) changes of receptor density (Bmax) with a minimum recorded at night. Binding of a constant amount of [125I]HEAT (200 pM) to pineal membranes at 8 time points exhibited a circadian variation (P < 0.001) of receptor density with lowest values around midnight and highest levels during daytime.

KW - Circadian variation

KW - Pineal gland

KW - α-Adrenoceptor

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