TY - JOUR
T1 - Tumor necrosis factor alpha (TNF-α) levels in the brain and cerebrospinal fluid after meningitis induced by Streptococcus pneumoniae
AU - Barichello, Tatiana
AU - dos Santos, Ivonete
AU - Savi, Geovana D.
AU - Florentino, Anelise F.
AU - Silvestre, Cintia
AU - Comim, Clarissa M.
AU - Feier, Gustavo
AU - Sachs, Daniela
AU - Teixeira, Mauro M.
AU - Teixeira, Antonio L.
AU - Quevedo, João
PY - 2009/12/31
Y1 - 2009/12/31
N2 - Bacterial meningitis due to Streptococcus pneumoniae is associated with a significant mortality rate and persisting neurologic sequelae including sensory-motor deficits, seizures, and impairments of learning and memory. The presence of proliferating bacteria within the subarachnoid and ventricular space compartments triggers an intense inflammatory host response at killing the invading microorganism. Proinflammatory mediators released in the process include tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6. TNF-α have several effects, including cytotoxicity, antiviral activity, transcription factor activation, and immune response regulation. Thus, the aim of this study was to verify the levels of the TNF-α after pneumococcal meningitis in male Wistar rats. The animals underwent a magna cistern tap receiving either 10 μL sterile saline as a placebo or an equivalent volume of a S. pneumoniae suspension at the concentration 5 × 109 cfu/mL. The animals were killed at 0, 6, 12, 24, 48 and 96 h after induction. The brain was removed and hippocampus, cortex, prefrontal and cerebrospinal fluid (CSF) were isolated and used for the determination of TNF-α levels. We found an increase in TNF-α levels at 6 h after induction of the meningitis in the hippocampus (p < 0.01), frontal cortex (p < 0.05), and cerebrospinal fluid (p < 0.001).There was no alteration in the cortex. Our data suggest that TNF-α is involved in the pathophysiology of the pneumococcal meningitis and could be investigated as a putative biomarker for brain damage in the first hours.
AB - Bacterial meningitis due to Streptococcus pneumoniae is associated with a significant mortality rate and persisting neurologic sequelae including sensory-motor deficits, seizures, and impairments of learning and memory. The presence of proliferating bacteria within the subarachnoid and ventricular space compartments triggers an intense inflammatory host response at killing the invading microorganism. Proinflammatory mediators released in the process include tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6. TNF-α have several effects, including cytotoxicity, antiviral activity, transcription factor activation, and immune response regulation. Thus, the aim of this study was to verify the levels of the TNF-α after pneumococcal meningitis in male Wistar rats. The animals underwent a magna cistern tap receiving either 10 μL sterile saline as a placebo or an equivalent volume of a S. pneumoniae suspension at the concentration 5 × 109 cfu/mL. The animals were killed at 0, 6, 12, 24, 48 and 96 h after induction. The brain was removed and hippocampus, cortex, prefrontal and cerebrospinal fluid (CSF) were isolated and used for the determination of TNF-α levels. We found an increase in TNF-α levels at 6 h after induction of the meningitis in the hippocampus (p < 0.01), frontal cortex (p < 0.05), and cerebrospinal fluid (p < 0.001).There was no alteration in the cortex. Our data suggest that TNF-α is involved in the pathophysiology of the pneumococcal meningitis and could be investigated as a putative biomarker for brain damage in the first hours.
KW - CNS
KW - Meningitis
KW - Streptococcus pneumoniae
KW - TNF-α
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U2 - 10.1016/j.neulet.2009.10.039
DO - 10.1016/j.neulet.2009.10.039
M3 - Article
C2 - 19835931
AN - SCOPUS:70449389158
SN - 0304-3940
VL - 467
SP - 217
EP - 219
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -