Tumor necrosis factor-alpha induces circular forms of human immunodeficiency virus type-1 DNA in the persistently infected low-level expressing cell line, ACH-2

Katsuhiko Kitamura, Sherif R. Zaki, Patricia W. Greer, Saswati D. Sinha, Thomas M. Folks

    Research output: Contribution to journalArticlepeer-review

    2 Scopus citations

    Abstract

    The low human immunodeficiency virus type-1 (HIV-1) expressing T-cell line, ACH-2, was used to investigate accumulation of the circular, extrachromosomal form of HIV DNA (HD) after tumor necrosis factor-alpha (TNF-α) induction. We chose the 2 long terminal repeat (LTR) circular form to analyze unintegrated HD by polymerase chain reaction (PCR), using primer pairs which flank the 2 LTR HD. Approximately a 10-fold increase in 2 LTR HD was detected intracellularly in the TNF-α-induced ACH-2 cells using an end point-dilution assay. To examine the cellular compartment location of the 2 LTR HD accumulation, ACH-2 cells were fractionated into cytoplasmic and nuclear components and further subjected to PCR. A 4- to 5-fold increase in the 2 LTR HD signal was observed in the nuclear fraction. These results indicate that unintegrated HD increases in a chronically infected cell line after TNF-α induction. This phenomenon, which previously had been observed only with acute infections, may offer insight into basic pathogenic mechanisms.

    Original languageEnglish (US)
    Pages (from-to)113-118
    Number of pages6
    JournalVirus Research
    Volume27
    Issue number2
    DOIs
    StatePublished - Feb 1993

    Keywords

    • Circular HIV DNA by TNF-α
    • In situ hybridization
    • Polymerase chain reaction

    ASJC Scopus subject areas

    • Cancer Research
    • Virology
    • Infectious Diseases

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