Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment

Qianghu Wang, Baoli Hu, Xin Hu, Hoon Kim, Massimo Squatrito, Lisa Scarpace, Ana C. deCarvalho, Sali Lyu, Pengping Li, Yan Li, Floris Barthel, Hee Jin Cho, Yu Hsi Lin, Nikunj Satani, Emmanuel Martinez-Ledesma, Siyuan Zheng, Edward Chang, Charles Etienne Gabriel Sauvé, Adriana Olar, Zheng D. Lan & 14 others Gaetano Finocchiaro, Joanna J. Phillips, Mitchel S. Berger, Konrad R. Gabrusiewicz, Guocan Wang, Eskil Eskilsson, Jian Hu, Tom Mikkelsen, Ronald A. DePinho, Florian Muller, Amy B. Heimberger, Erik P. Sulman, Do Hyun Nam, Roel G.W. Verhaak

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

We leveraged IDH wild-type glioblastomas, derivative neurospheres, and single-cell gene expression profiles to define three tumor-intrinsic transcriptional subtypes designated as proneural, mesenchymal, and classical. Transcriptomic subtype multiplicity correlated with increased intratumoral heterogeneity and presence of tumor microenvironment. In silico cell sorting identified macrophages/microglia, CD4+ T lymphocytes, and neutrophils in the glioma microenvironment. NF1 deficiency resulted in increased tumor-associated macrophages/microglia infiltration. Longitudinal transcriptome analysis showed that expression subtype is retained in 55% of cases. Gene signature-based tumor microenvironment inference revealed a decrease in invading monocytes and a subtype-dependent increase in macrophages/microglia cells upon disease recurrence. Hypermutation at diagnosis or at recurrence associated with CD8+ T cell enrichment. Frequency of M2 macrophages detection associated with short-term relapse after radiation therapy. Wang et al. define three IDH wild-type glioblastoma-intrinsic gene expression subtypes, which are partly shaped by the tumor immune environment. NF1 deficiency results in increased macrophage/microglia infiltration. Comparison of matched primary and recurrent tumors reveals frequent expression subtype changes.

Original languageEnglish (US)
Pages (from-to)42-56.e6
JournalCancer Cell
Volume32
Issue number1
DOIs
StatePublished - Jul 10 2017
Externally publishedYes

Fingerprint

Glioma
Microglia
Macrophages
Gene Expression
Tumor Microenvironment
Neoplasms
Glioblastoma
Recurrence
T-Lymphocytes
Gene Expression Profiling
Transcriptome
Computer Simulation
Monocytes
Neutrophils
Radiotherapy
Genes

Keywords

  • disease recurrence
  • glioblastoma
  • immune cells
  • macrophages/microglia
  • mesenchymal subtype
  • proneural to mesenchymal transition
  • tumor evolution
  • tumor microenvironment

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

Cite this

Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment. / Wang, Qianghu; Hu, Baoli; Hu, Xin; Kim, Hoon; Squatrito, Massimo; Scarpace, Lisa; deCarvalho, Ana C.; Lyu, Sali; Li, Pengping; Li, Yan; Barthel, Floris; Cho, Hee Jin; Lin, Yu Hsi; Satani, Nikunj; Martinez-Ledesma, Emmanuel; Zheng, Siyuan; Chang, Edward; Sauvé, Charles Etienne Gabriel; Olar, Adriana; Lan, Zheng D.; Finocchiaro, Gaetano; Phillips, Joanna J.; Berger, Mitchel S.; Gabrusiewicz, Konrad R.; Wang, Guocan; Eskilsson, Eskil; Hu, Jian; Mikkelsen, Tom; DePinho, Ronald A.; Muller, Florian; Heimberger, Amy B.; Sulman, Erik P.; Nam, Do Hyun; Verhaak, Roel G.W.

In: Cancer Cell, Vol. 32, No. 1, 10.07.2017, p. 42-56.e6.

Research output: Contribution to journalArticle

Wang, Q, Hu, B, Hu, X, Kim, H, Squatrito, M, Scarpace, L, deCarvalho, AC, Lyu, S, Li, P, Li, Y, Barthel, F, Cho, HJ, Lin, YH, Satani, N, Martinez-Ledesma, E, Zheng, S, Chang, E, Sauvé, CEG, Olar, A, Lan, ZD, Finocchiaro, G, Phillips, JJ, Berger, MS, Gabrusiewicz, KR, Wang, G, Eskilsson, E, Hu, J, Mikkelsen, T, DePinho, RA, Muller, F, Heimberger, AB, Sulman, EP, Nam, DH & Verhaak, RGW 2017, 'Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment', Cancer Cell, vol. 32, no. 1, pp. 42-56.e6. https://doi.org/10.1016/j.ccell.2017.06.003
Wang, Qianghu ; Hu, Baoli ; Hu, Xin ; Kim, Hoon ; Squatrito, Massimo ; Scarpace, Lisa ; deCarvalho, Ana C. ; Lyu, Sali ; Li, Pengping ; Li, Yan ; Barthel, Floris ; Cho, Hee Jin ; Lin, Yu Hsi ; Satani, Nikunj ; Martinez-Ledesma, Emmanuel ; Zheng, Siyuan ; Chang, Edward ; Sauvé, Charles Etienne Gabriel ; Olar, Adriana ; Lan, Zheng D. ; Finocchiaro, Gaetano ; Phillips, Joanna J. ; Berger, Mitchel S. ; Gabrusiewicz, Konrad R. ; Wang, Guocan ; Eskilsson, Eskil ; Hu, Jian ; Mikkelsen, Tom ; DePinho, Ronald A. ; Muller, Florian ; Heimberger, Amy B. ; Sulman, Erik P. ; Nam, Do Hyun ; Verhaak, Roel G.W. / Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment. In: Cancer Cell. 2017 ; Vol. 32, No. 1. pp. 42-56.e6.
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AU - Scarpace, Lisa

AU - deCarvalho, Ana C.

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AU - Barthel, Floris

AU - Cho, Hee Jin

AU - Lin, Yu Hsi

AU - Satani, Nikunj

AU - Martinez-Ledesma, Emmanuel

AU - Zheng, Siyuan

AU - Chang, Edward

AU - Sauvé, Charles Etienne Gabriel

AU - Olar, Adriana

AU - Lan, Zheng D.

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AU - Berger, Mitchel S.

AU - Gabrusiewicz, Konrad R.

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AU - Eskilsson, Eskil

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AU - Mikkelsen, Tom

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AU - Nam, Do Hyun

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KW - tumor evolution

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