Tubular hypertrophy due to work load induced by furosemide is associated with increases of IGF-1 and IGFBP-1

S. Kobayashi, D. R. Clemmons, H. Nogami, A. K. Roy, Manjeri A Venkatachalam

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We have examined the expression of insulin-like growth factor 1 (IGF-1), IGF binding protein-1 (IGFBP-1), and IGF binding protein-3 (IGFBP-3) in the rat distal nephron during increased cell work load and hypertrophy, induced by the diuretic, furosemide. Furosemide was given for six days to increase distal sodium delivery and uptake. To mitigate salt loss, the animals drank 0.8% NaCl and 0.1% KCl. Control rats were infused with vehicle (0.9% saline) and drank tap water. Furosemide increased urinary volume (13-fold) and sodium excretion (eightfold), and decreased urine osmolarity (fourfold). By immunocytochemistry, staining for IGF-1 and IGFBP-1 was markedly increased in distal convoluted tubules and cortical collecting ducts; both segments also underwent hypertrophy. Increased staining for the peptides was evident early (1 hr, 18 hr) after furosemide, prior to hypertrophy of cells. Whereas transcripts of IGF-1 and IGFBP-3 mRNA showed little or no increase in extracts from furosemide-treated kidney cortices, IGFBP-1 mRNA was increased threefold 18 hours after furosemide. Alterations of IGF-1 and IGFBP-1 were independent of changes in plasma aldosterone, glucocorticoids or arginine vasopressin. That IGFBP-1 mRNA increased threefold without significant changes in IGF-1 mRNA suggests that hypertrophic stimuli might initially induce the synthesis of IGF binding protein followed by the trapping of extracellular IGF-1. The present study raises the possibility of IGF-1 and IGFBP-1 being involved in processes that lead to tubular hypertrophy. IGFBP-1 may regulate these effects by binding to and interaction with IGF-1.

Original languageEnglish (US)
Pages (from-to)818-828
Number of pages11
JournalKidney International
Volume47
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Protein 1
Furosemide
Somatomedins
Workload
Hypertrophy
Messenger RNA
Insulin-Like Growth Factor Binding Protein 3
Sodium
Staining and Labeling
Kidney Cortex
Insulin-Like Growth Factor Binding Proteins
Arginine Vasopressin
Nephrons
Aldosterone
Diuretics
Osmolar Concentration
Glucocorticoids
Salts
Immunohistochemistry
Urine

ASJC Scopus subject areas

  • Nephrology

Cite this

Tubular hypertrophy due to work load induced by furosemide is associated with increases of IGF-1 and IGFBP-1. / Kobayashi, S.; Clemmons, D. R.; Nogami, H.; Roy, A. K.; Venkatachalam, Manjeri A.

In: Kidney International, Vol. 47, No. 3, 1995, p. 818-828.

Research output: Contribution to journalArticle

Kobayashi, S. ; Clemmons, D. R. ; Nogami, H. ; Roy, A. K. ; Venkatachalam, Manjeri A. / Tubular hypertrophy due to work load induced by furosemide is associated with increases of IGF-1 and IGFBP-1. In: Kidney International. 1995 ; Vol. 47, No. 3. pp. 818-828.
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