Tubercidin stabilizes microtubules against vinblastine-induced depolymerization, a taxol-like effect

Susan L. Mooberry; Klemens Stratman; Richard E. Moore

doi:10.1016/0304-3835(95)03940-X

Tubercidin stabilizes microtubules against vinblastine-induced depolymerization, a taxol-like effect

Susan L. Mooberry, Klemens Stratman, Richard E. Moore

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A sensitive assay for the detection of microtubule-stabilizing agents [1] was used to screen an extensive collection of cyanobacterial and microalgal extracts. The hydrophilic extract of the cyanobacterium, Plectonema radiosum (UH isolate IC-70-1), exhibited microtubule-stabilizing activity. Bioassay-directed purification of the active compound yielded tubercidin (7-deazaadenosine), a potent cytotoxic nucleoside analog. Further studies revealed that tubercidin protected a population of cellular microtubules against vinblastine-induced depolymerization, a microtubule-stabilizing, taxol-like effect. The microtubule-stabilizing effect of tubercidin is dose dependent and limited by the cytotoxicity of the agent. Tubercidin represents another natural product that interacts with microtubules and is one of the few to cause microtubule stabilization.

Original language	English (US)
Pages (from-to)	261-266
Number of pages	6
Journal	Cancer Letters
Volume	96
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(95)03940-X
State	Published - Sep 25 1995
Externally published	Yes

Keywords

Cancer
Cyanobacteria
Microtubule
Taxol
Tubercidin

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(95)03940-X

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title = "Tubercidin stabilizes microtubules against vinblastine-induced depolymerization, a taxol-like effect",

abstract = "A sensitive assay for the detection of microtubule-stabilizing agents [1] was used to screen an extensive collection of cyanobacterial and microalgal extracts. The hydrophilic extract of the cyanobacterium, Plectonema radiosum (UH isolate IC-70-1), exhibited microtubule-stabilizing activity. Bioassay-directed purification of the active compound yielded tubercidin (7-deazaadenosine), a potent cytotoxic nucleoside analog. Further studies revealed that tubercidin protected a population of cellular microtubules against vinblastine-induced depolymerization, a microtubule-stabilizing, taxol-like effect. The microtubule-stabilizing effect of tubercidin is dose dependent and limited by the cytotoxicity of the agent. Tubercidin represents another natural product that interacts with microtubules and is one of the few to cause microtubule stabilization.",

keywords = "Cancer, Cyanobacteria, Microtubule, Taxol, Tubercidin",

author = "Mooberry, {Susan L.} and Klemens Stratman and Moore, {Richard E.}",

note = "Funding Information: This work was supported in part by the State of Hawaii, Office of Technology Transfer and Economic Development (SLM), an American Cancer Society Institutional Research Grant IRG-190 (SLM) and NM Grant CA-12623 (REM).",

year = "1995",

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doi = "10.1016/0304-3835(95)03940-X",

language = "English (US)",

volume = "96",

pages = "261--266",

journal = "Cancer Letters",

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T1 - Tubercidin stabilizes microtubules against vinblastine-induced depolymerization, a taxol-like effect

AU - Mooberry, Susan L.

AU - Stratman, Klemens

AU - Moore, Richard E.

N1 - Funding Information: This work was supported in part by the State of Hawaii, Office of Technology Transfer and Economic Development (SLM), an American Cancer Society Institutional Research Grant IRG-190 (SLM) and NM Grant CA-12623 (REM).

PY - 1995/9/25

Y1 - 1995/9/25

N2 - A sensitive assay for the detection of microtubule-stabilizing agents [1] was used to screen an extensive collection of cyanobacterial and microalgal extracts. The hydrophilic extract of the cyanobacterium, Plectonema radiosum (UH isolate IC-70-1), exhibited microtubule-stabilizing activity. Bioassay-directed purification of the active compound yielded tubercidin (7-deazaadenosine), a potent cytotoxic nucleoside analog. Further studies revealed that tubercidin protected a population of cellular microtubules against vinblastine-induced depolymerization, a microtubule-stabilizing, taxol-like effect. The microtubule-stabilizing effect of tubercidin is dose dependent and limited by the cytotoxicity of the agent. Tubercidin represents another natural product that interacts with microtubules and is one of the few to cause microtubule stabilization.

AB - A sensitive assay for the detection of microtubule-stabilizing agents [1] was used to screen an extensive collection of cyanobacterial and microalgal extracts. The hydrophilic extract of the cyanobacterium, Plectonema radiosum (UH isolate IC-70-1), exhibited microtubule-stabilizing activity. Bioassay-directed purification of the active compound yielded tubercidin (7-deazaadenosine), a potent cytotoxic nucleoside analog. Further studies revealed that tubercidin protected a population of cellular microtubules against vinblastine-induced depolymerization, a microtubule-stabilizing, taxol-like effect. The microtubule-stabilizing effect of tubercidin is dose dependent and limited by the cytotoxicity of the agent. Tubercidin represents another natural product that interacts with microtubules and is one of the few to cause microtubule stabilization.

KW - Cancer

KW - Cyanobacteria

KW - Microtubule

KW - Taxol

KW - Tubercidin

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AN - SCOPUS:0029150213

VL - 96

SP - 261

EP - 266

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 2

ER -

Tubercidin stabilizes microtubules against vinblastine-induced depolymerization, a taxol-like effect

Abstract

Keywords

ASJC Scopus subject areas

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