Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta

Guillermina Laura Dolcini, María Elisa Solana, Guadalupe Andreani, Ana María Celentano, Laura María Parodi, Ana María Donato, Natalia Elissondo, Stella Maris González Cappa, Luis David Giavedoni, Liliana Martínez Peralta

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Several factors determine the risk of HIV mother-to-child transmission (MTCT), such as coinfections in placentas from HIV-1 positive mothers with other pathogens. Chagas' disease is one of the most endemic zoonoses in Latin America, caused by the protozoan Trypanosoma cruzi. The purpose of the study was to determine whether T. cruzi modifies HIV infection of the placenta at the tissue or cellular level. Results: Simple and double infections were carried out on a placental histoculture system (chorionic villi isolated from term placentas from HIV and Chagas negative mothers) and on the choriocarcinoma BeWo cell line. Trypomastigotes of T. cruzi (VD lethal strain), either purified from mouse blood or from Vero cell cultures, 24 h-supernatants of blood and cellular trypomastigotes, and the VSV-G pseudotyped HIV-1 reporter virus were used for the coinfections. Viral transduction was evaluated by quantification of luciferase activity. Coinfection with whole trypomastigotes, either from mouse blood or from cell cultures, decreased viral pseudotype luciferase activity in placental histocultures. Similar results were obtained from BeWo cells. Supernatants of stimulated histocultures were used for the simultaneous determination of 29 cytokines and chemokines with the Luminex technology. In histocultures infected with trypomastigotes, as well as in coinfected tissues, IL-6, IL-8, IP-10 and MCP-1 production was significantly lower than in controls or HIV-1 transducted tissue. A similar decrease was observed in histocultures treated with 24 h-supernatants of blood trypomastigotes, but not in coinfected tissues. Conclusion: Our results demonstrated that the presence of an intracellular pathogen, such as T. cruzi, is able to impair HIV-1 transduction in an in vitro system of human placental histoculture. Direct effects of the parasite on cellular structures as well as on cellular/viral proteins essential for HIV-1 replication might influence viral transduction in this model. Nonetheless, additional mechanisms including modulation of cytokines/chemokines at placental level could not be excluded in the inhibition observed. Further experiments need to be conducted in order to elucidate the mechanism(s) involved in this phenomenon. Therefore, coinfection with T. cruzi may have a deleterious effect on HIV-1 transduction and thus could play an important role in viral outcome at the placental level.

Original languageEnglish (US)
Article number53
JournalRetrovirology
Volume5
DOIs
StatePublished - Jul 1 2008
Externally publishedYes

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Chagas Disease
Trypanosoma cruzi
Placenta
HIV-1
Coinfection
Mothers
Luciferases
Chemokines
Cell Culture Techniques
HIV
Cytokines
Chorionic Villi
Choriocarcinoma
Vero Cells
Latin America
Zoonoses
Viral Proteins
Cellular Structures
Interleukin-8
HIV Infections

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Dolcini, G. L., Solana, M. E., Andreani, G., Celentano, A. M., Parodi, L. M., Donato, A. M., ... Peralta, L. M. (2008). Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta. Retrovirology, 5, [53]. https://doi.org/10.1186/1742-4690-5-53

Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta. / Dolcini, Guillermina Laura; Solana, María Elisa; Andreani, Guadalupe; Celentano, Ana María; Parodi, Laura María; Donato, Ana María; Elissondo, Natalia; Cappa, Stella Maris González; Giavedoni, Luis David; Peralta, Liliana Martínez.

In: Retrovirology, Vol. 5, 53, 01.07.2008.

Research output: Contribution to journalArticle

Dolcini, GL, Solana, ME, Andreani, G, Celentano, AM, Parodi, LM, Donato, AM, Elissondo, N, Cappa, SMG, Giavedoni, LD & Peralta, LM 2008, 'Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta', Retrovirology, vol. 5, 53. https://doi.org/10.1186/1742-4690-5-53
Dolcini GL, Solana ME, Andreani G, Celentano AM, Parodi LM, Donato AM et al. Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta. Retrovirology. 2008 Jul 1;5. 53. https://doi.org/10.1186/1742-4690-5-53
Dolcini, Guillermina Laura ; Solana, María Elisa ; Andreani, Guadalupe ; Celentano, Ana María ; Parodi, Laura María ; Donato, Ana María ; Elissondo, Natalia ; Cappa, Stella Maris González ; Giavedoni, Luis David ; Peralta, Liliana Martínez. / Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta. In: Retrovirology. 2008 ; Vol. 5.
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AU - Celentano, Ana María

AU - Parodi, Laura María

AU - Donato, Ana María

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