Treponema pallidum receptor binding proteins interact with fibronectin

K. M. Peterson, J. B. Baseman, J. F. Alderete

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Analysis of plasma proteins avidly bound to T. pallidum surfaces revealed the ability of T. pallidum to acquire numerous host macromolecules. No acquisition was evident by the avirulent spirochete, T. phagendenis biotype Reiter. Western blotting technology using hyperimmune antifibronectin serum as a probe revealed the ability of virulent treponemes to avidly bind fibronectin from a complex medium such as plasma. The specificity of the tiplike adherence of motile T. pallidum to fibronectin-coated glass surfaces and to fibronectin on HE(p)-2 cells was reinforced by the observation that pretreatment of coverslips or cell monolayers with monospecific antiserum against fibronectin substantially reduced T. pallidum attachment. The stoichiometric binding of T. pallidum to fibronectin-coated coverslips and the inability of unlabeled or 35S-radiolabeled treponemes to interact with glass surfaces treated with other plasma proteins further established the specific nature of the interaction between virulent T. pallidum and fibronectin. The avid association between three outer envelope proteins of T. pallidum and fibronectin was also demonstrated. These treponemal surface proteins have been previously identified as putative receptor-binding proteins responsible for T. pallidum parasitism of host cells. The data suggest that surface fibronectin mediates tip-oriented attachment of T. pallidum to host cells via a receptor-ligand mechanism of recognition.

Original languageEnglish (US)
Pages (from-to)1958-1970
Number of pages13
JournalJournal of Experimental Medicine
Volume157
Issue number6
StatePublished - 1983

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Treponema pallidum
Fibronectins
Carrier Proteins
Glass
Blood Proteins
Spirochaetales
Immune Sera
Membrane Proteins
Western Blotting
Ligands
Technology

ASJC Scopus subject areas

  • Immunology

Cite this

Peterson, K. M., Baseman, J. B., & Alderete, J. F. (1983). Treponema pallidum receptor binding proteins interact with fibronectin. Journal of Experimental Medicine, 157(6), 1958-1970.

Treponema pallidum receptor binding proteins interact with fibronectin. / Peterson, K. M.; Baseman, J. B.; Alderete, J. F.

In: Journal of Experimental Medicine, Vol. 157, No. 6, 1983, p. 1958-1970.

Research output: Contribution to journalArticle

Peterson, KM, Baseman, JB & Alderete, JF 1983, 'Treponema pallidum receptor binding proteins interact with fibronectin', Journal of Experimental Medicine, vol. 157, no. 6, pp. 1958-1970.
Peterson, K. M. ; Baseman, J. B. ; Alderete, J. F. / Treponema pallidum receptor binding proteins interact with fibronectin. In: Journal of Experimental Medicine. 1983 ; Vol. 157, No. 6. pp. 1958-1970.
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