Trauma-hemorrhage induces depressed splenic dendritic cell functions in mice

Takashi Kawasaki, William J. Hubbard, Mashkoor A. Choudhry, Martin G Schwacha, Kirby I. Bland, Irshad H. Chaudry

Research output: Contribution to journalArticle

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Abstract

Although Kupffer cell, splenic, and peritoneal macrophage functions are markedly altered following trauma-hemorrhage (T-H), it remains unclear whether T-H also affects splenic dendritic cell (sDC) functions. We hypothesized that sDC functions will also be compromised following T-H. Male C3H/HeN (6- to 8-wk) mice were randomly assigned to sham operation or T-H. T-H was induced by midline laparotomy and ∼90 min of hemorrhagic shock (blood pressure 35 mmHg), followed by fluid resuscitation (four times the shed blood volume in the form of Ringer's lactate). Two hours later, the mice were sacrificed; sDC were isolated; and the changes in their apoptosis, MHC class II expression, and ability to produce costimulatory cytokines and Ag presentation were measured. The results indicate that sDC Ag presentation capacity was significantly decreased and MHC class II expression was also significantly decreased following T-H. Moreover, LPS-induced IL-12 production and LPS- or IL-12-induced IFN-γ production following T-H were significantly decreased. Thus, the markedly decreased M-H class II expression and cytokine (IL-12, IFN-γ) production following T-H may be the cause for the depressed sDC Ag presentation under those conditions. This depression in Ag presentation could contribute to the host's enhanced susceptibility to sepsis following T-H.

Original languageEnglish (US)
Pages (from-to)4514-4520
Number of pages7
JournalJournal of Immunology
Volume177
Issue number7
StatePublished - Oct 1 2006
Externally publishedYes

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Dendritic Cells
Hemorrhage
Interleukin-12
Wounds and Injuries
Cytokines
Kupffer Cells
Hemorrhagic Shock
Peritoneal Macrophages
Blood Volume
Resuscitation
Laparotomy
Sepsis
Apoptosis
Blood Pressure

ASJC Scopus subject areas

  • Immunology

Cite this

Kawasaki, T., Hubbard, W. J., Choudhry, M. A., Schwacha, M. G., Bland, K. I., & Chaudry, I. H. (2006). Trauma-hemorrhage induces depressed splenic dendritic cell functions in mice. Journal of Immunology, 177(7), 4514-4520.

Trauma-hemorrhage induces depressed splenic dendritic cell functions in mice. / Kawasaki, Takashi; Hubbard, William J.; Choudhry, Mashkoor A.; Schwacha, Martin G; Bland, Kirby I.; Chaudry, Irshad H.

In: Journal of Immunology, Vol. 177, No. 7, 01.10.2006, p. 4514-4520.

Research output: Contribution to journalArticle

Kawasaki, T, Hubbard, WJ, Choudhry, MA, Schwacha, MG, Bland, KI & Chaudry, IH 2006, 'Trauma-hemorrhage induces depressed splenic dendritic cell functions in mice', Journal of Immunology, vol. 177, no. 7, pp. 4514-4520.
Kawasaki T, Hubbard WJ, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH. Trauma-hemorrhage induces depressed splenic dendritic cell functions in mice. Journal of Immunology. 2006 Oct 1;177(7):4514-4520.
Kawasaki, Takashi ; Hubbard, William J. ; Choudhry, Mashkoor A. ; Schwacha, Martin G ; Bland, Kirby I. ; Chaudry, Irshad H. / Trauma-hemorrhage induces depressed splenic dendritic cell functions in mice. In: Journal of Immunology. 2006 ; Vol. 177, No. 7. pp. 4514-4520.
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