Transport of cholesterol across a BeWo cell monolayer: Implications for net transport of sterol from maternal to fetal circulation

Kara E. Schmid, W. Sean Davidson, Leslie Myatt, Laura A. Woollett

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The placental transport of various compounds, such as glucose and fatty acids, has been well studied. However, the transport of cholesterol, a sterol essential for proper fetal development, remains undefined in the placenta. Therefore, the purpose of these studies was to examine the transport of cholesterol across a placental monolayer and its uptake by various cholesterol acceptors. BeWo cells, which originated from a human choriocarcinoma, were grown on transwells for 3 days to form a confluent monolayer. The apical side of the cells was radiolabeled with either free cholesterol or LDL cholesteryl ester. After 24 h, the radiolabel was removed and cholesterol acceptors were added to the basolateral chamber. Cholesterol was found to be taken up by the apical surface of the placental monolayer, transported to the basolateral surface of the cell, and effluxed to fetal human serum, fetal HDL, or phospholipid vesicles, but not to apolipoprotein A-I. In addition, increasing the cellular cholesterol concentration further increased the amount of cholesterol transported to the basolateral acceptors. These are the first studies to demonstrate the movement of cholesterol across a placental cell from the maternal circulation (apical side) to the fetal circulation (basolateral side).

Original languageEnglish (US)
Pages (from-to)1909-1918
Number of pages10
JournalJournal of Lipid Research
Volume44
Issue number10
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Sterols
Monolayers
Cholesterol
Mothers
Choriocarcinoma
Cholesterol Esters
Apolipoprotein A-I
Fetal Development
LDL Cholesterol
Placenta
Phospholipids
Fatty Acids
Glucose
Serum

Keywords

  • Cholesterol efflux
  • Fetus
  • Low density lipoprotein
  • Placenta
  • Smith-Lemli-Opitz syndrome

ASJC Scopus subject areas

  • Endocrinology

Cite this

Transport of cholesterol across a BeWo cell monolayer : Implications for net transport of sterol from maternal to fetal circulation. / Schmid, Kara E.; Davidson, W. Sean; Myatt, Leslie; Woollett, Laura A.

In: Journal of Lipid Research, Vol. 44, No. 10, 10.2003, p. 1909-1918.

Research output: Contribution to journalArticle

Schmid, Kara E. ; Davidson, W. Sean ; Myatt, Leslie ; Woollett, Laura A. / Transport of cholesterol across a BeWo cell monolayer : Implications for net transport of sterol from maternal to fetal circulation. In: Journal of Lipid Research. 2003 ; Vol. 44, No. 10. pp. 1909-1918.
@article{c53a7b11b8a945a5b183f92613243cc2,
title = "Transport of cholesterol across a BeWo cell monolayer: Implications for net transport of sterol from maternal to fetal circulation",
abstract = "The placental transport of various compounds, such as glucose and fatty acids, has been well studied. However, the transport of cholesterol, a sterol essential for proper fetal development, remains undefined in the placenta. Therefore, the purpose of these studies was to examine the transport of cholesterol across a placental monolayer and its uptake by various cholesterol acceptors. BeWo cells, which originated from a human choriocarcinoma, were grown on transwells for 3 days to form a confluent monolayer. The apical side of the cells was radiolabeled with either free cholesterol or LDL cholesteryl ester. After 24 h, the radiolabel was removed and cholesterol acceptors were added to the basolateral chamber. Cholesterol was found to be taken up by the apical surface of the placental monolayer, transported to the basolateral surface of the cell, and effluxed to fetal human serum, fetal HDL, or phospholipid vesicles, but not to apolipoprotein A-I. In addition, increasing the cellular cholesterol concentration further increased the amount of cholesterol transported to the basolateral acceptors. These are the first studies to demonstrate the movement of cholesterol across a placental cell from the maternal circulation (apical side) to the fetal circulation (basolateral side).",
keywords = "Cholesterol efflux, Fetus, Low density lipoprotein, Placenta, Smith-Lemli-Opitz syndrome",
author = "Schmid, {Kara E.} and Davidson, {W. Sean} and Leslie Myatt and Woollett, {Laura A.}",
year = "2003",
month = "10",
doi = "10.1194/jlr.M300126-JLR200",
language = "English (US)",
volume = "44",
pages = "1909--1918",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "10",

}

TY - JOUR

T1 - Transport of cholesterol across a BeWo cell monolayer

T2 - Implications for net transport of sterol from maternal to fetal circulation

AU - Schmid, Kara E.

AU - Davidson, W. Sean

AU - Myatt, Leslie

AU - Woollett, Laura A.

PY - 2003/10

Y1 - 2003/10

N2 - The placental transport of various compounds, such as glucose and fatty acids, has been well studied. However, the transport of cholesterol, a sterol essential for proper fetal development, remains undefined in the placenta. Therefore, the purpose of these studies was to examine the transport of cholesterol across a placental monolayer and its uptake by various cholesterol acceptors. BeWo cells, which originated from a human choriocarcinoma, were grown on transwells for 3 days to form a confluent monolayer. The apical side of the cells was radiolabeled with either free cholesterol or LDL cholesteryl ester. After 24 h, the radiolabel was removed and cholesterol acceptors were added to the basolateral chamber. Cholesterol was found to be taken up by the apical surface of the placental monolayer, transported to the basolateral surface of the cell, and effluxed to fetal human serum, fetal HDL, or phospholipid vesicles, but not to apolipoprotein A-I. In addition, increasing the cellular cholesterol concentration further increased the amount of cholesterol transported to the basolateral acceptors. These are the first studies to demonstrate the movement of cholesterol across a placental cell from the maternal circulation (apical side) to the fetal circulation (basolateral side).

AB - The placental transport of various compounds, such as glucose and fatty acids, has been well studied. However, the transport of cholesterol, a sterol essential for proper fetal development, remains undefined in the placenta. Therefore, the purpose of these studies was to examine the transport of cholesterol across a placental monolayer and its uptake by various cholesterol acceptors. BeWo cells, which originated from a human choriocarcinoma, were grown on transwells for 3 days to form a confluent monolayer. The apical side of the cells was radiolabeled with either free cholesterol or LDL cholesteryl ester. After 24 h, the radiolabel was removed and cholesterol acceptors were added to the basolateral chamber. Cholesterol was found to be taken up by the apical surface of the placental monolayer, transported to the basolateral surface of the cell, and effluxed to fetal human serum, fetal HDL, or phospholipid vesicles, but not to apolipoprotein A-I. In addition, increasing the cellular cholesterol concentration further increased the amount of cholesterol transported to the basolateral acceptors. These are the first studies to demonstrate the movement of cholesterol across a placental cell from the maternal circulation (apical side) to the fetal circulation (basolateral side).

KW - Cholesterol efflux

KW - Fetus

KW - Low density lipoprotein

KW - Placenta

KW - Smith-Lemli-Opitz syndrome

UR - http://www.scopus.com/inward/record.url?scp=0242322476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242322476&partnerID=8YFLogxK

U2 - 10.1194/jlr.M300126-JLR200

DO - 10.1194/jlr.M300126-JLR200

M3 - Article

C2 - 12897187

AN - SCOPUS:0242322476

VL - 44

SP - 1909

EP - 1918

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 10

ER -