Translational requirement of La Crosse virus S-mRNA synthesis: In vitro studies

C. Bellocq, R. Raju, J. Patterson, D. Kolakofsky

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The exceptional requirement of La Crosse virus mRNA synthesis for ongoing protein synthesis in vivo was examined in vitro by using purified virions and a reticulocyte lysate. Transcription from the S genome produced two incomplete transcripts (110 and 205 nucleotides [nt]) in the absence of the lysate, whereas S-mRNA (900 nt) was predominantly made when the lysate was present. The addition of drugs which inhibit protein synthesis also inhibited the synthesis of S-mRNA, and in some cases led to the reappearance of the 205-nt RNA. Reconstruction experiments demonstrated that the incomplete transcripts were not the result of rapid and selective degradation of S-mRNA but were due to premature termination of the polymerase at defined sites. The requirement for ongoing protein synthesis for productive transcription in vitro is not at the level of chain initiation but for elongation of the nascent RNA beyond these sites.

Original languageEnglish (US)
Pages (from-to)87-95
Number of pages9
JournalJournal of Virology
Volume61
Issue number1
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Bellocq, C., Raju, R., Patterson, J., & Kolakofsky, D. (1987). Translational requirement of La Crosse virus S-mRNA synthesis: In vitro studies. Journal of Virology, 61(1), 87-95.