Translational and HIF-1α-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides

Laura Hulea, Simon Pierre Gravel, Masahiro Morita, Marie Cargnello, Oro Uchenunu, Young Kyuen Im, Camille Lehuédé, Eric H. Ma, Matthew Leibovitch, Shannon McLaughlan, Marie José Blouin, Maxime Parisotto, Vasilios Papavasiliou, Cynthia Lavoie, Ola Larsson, Michael Ohh, Tiago Ferreira, Celia Greenwood, Gaëlle Bridon, Daina AvizonisGerardo Ferbeyre, Peter Siegel, Russell G. Jones, William Muller, Josie Ursini-Siegel, Julie St-Pierre, Michael Pollak, Ivan Topisirovic

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

There is increasing interest in therapeutically exploiting metabolic differences between normal and cancer cells. We show that kinase inhibitors (KIs) and biguanides synergistically and selectively target a variety of cancer cells. Synthesis of non-essential amino acids (NEAAs) aspartate, asparagine, and serine, as well as glutamine metabolism, are major determinants of the efficacy of KI/biguanide combinations. The mTORC1/4E-BP axis regulates aspartate, asparagine, and serine synthesis by modulating mRNA translation, while ablation of 4E-BP1/2 substantially decreases sensitivity of breast cancer and melanoma cells to KI/biguanide combinations. Efficacy of the KI/biguanide combinations is also determined by HIF-1α-dependent perturbations in glutamine metabolism, which were observed in VHL-deficient renal cancer cells. This suggests that cancer cells display metabolic plasticity by engaging non-redundant adaptive mechanisms, which allows them to survive therapeutic insults that target cancer metabolism.

Original languageEnglish (US)
Pages (from-to)817-832.e8
JournalCell Metabolism
Volume28
Issue number6
DOIs
StatePublished - Dec 4 2018

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Keywords

  • biguanide
  • cancer
  • HIF-1α
  • kinase inhibitor
  • metabolic plasticity
  • metabolism
  • mRNA translation
  • mTORC1
  • non-essential amino acids

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Hulea, L., Gravel, S. P., Morita, M., Cargnello, M., Uchenunu, O., Im, Y. K., Lehuédé, C., Ma, E. H., Leibovitch, M., McLaughlan, S., Blouin, M. J., Parisotto, M., Papavasiliou, V., Lavoie, C., Larsson, O., Ohh, M., Ferreira, T., Greenwood, C., Bridon, G., ... Topisirovic, I. (2018). Translational and HIF-1α-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides. Cell Metabolism, 28(6), 817-832.e8. https://doi.org/10.1016/j.cmet.2018.09.001