Transfusion-related acute lung injury in a rat model of trauma-hemorrhage

Susannah E. Nicholson, Robert A. Johnson, Teresa Craig, John G Myers, William Durante, Ronald M Stewart, Fruzsina K. Johnson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Major trauma often causes hemorrhage and predisposes to transfusion-related acute lung injury (TRALI). TRALI is a leading cause of transfusion-related deaths; however, its pathophysiology is uncertain. In the existing two-event models of TRALI, infection (lipopolysaccharide injection) is followed by the infusion of aged blood products. Our objective was to develop a trauma-relevant two-event model of TRALI by examining the effect of aged packed red blood cells (PRBC) on lung injury in rats with trauma-hemorrhage. Methods: Male Lewis rats were used. Rat PRBC were prepared similar to human PRBC. Recipients were implanted with femoral arterial and venous catheters (isoflurane anesthesia) and then subjected to 30% controlled arterial hemorrhage after 16-hour recovery. After a 60-minute shock period, rats were resuscitated with crystalloid and PRBC (0-35 days old; 3:1 ratio) and followed for up to 6 hours. Lung edema was evaluated by Evans blue dye (EBD), protein, and cytokine-induced neutrophil chemoattractant-1 (CINC-1) accumulation in bronchoalveolar lavage fluid, and arterial blood gases were measured (iSTAT). Results: CINC-1 levels increased over time in our PRBC stored for over 21 days. Transfusion survival was reduced, and Evans blue dye, protein, and CINC-1 accumulation in bronchoalveolar lavage fluid were increased in rats transfused with 28-day-old and 35-day-old PRBC compared with the 0-day group. Arterial Po2 and O2 saturation were decreased in rats transfused with 28-day-old and 35-day-old PRBC. However, pH and Pco2 were not different between groups. CONCLUSIONS:: These results suggest that transfusion of 28-day-old and 35-day-old PRBC reliably promotes lung edema in a rat model of catheter surgery and hemorrhage. We propose that this model can be used as a trauma-relevant two-event moel of TRALI.

Original languageEnglish (US)
Pages (from-to)466-471
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume70
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Acute Lung Injury
Erythrocytes
Hemorrhage
Wounds and Injuries
Chemotactic Factors
Evans Blue
Neutrophils
Bronchoalveolar Lavage Fluid
Cytokines
Edema
Coloring Agents
Catheters
Lung
Isoflurane
Lung Injury
Thigh
Lipopolysaccharides
Shock
Proteins
Anesthesia

Keywords

  • Animal model
  • Hemorrhage
  • Lung injury
  • Transfusion
  • Trauma

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

Cite this

Transfusion-related acute lung injury in a rat model of trauma-hemorrhage. / Nicholson, Susannah E.; Johnson, Robert A.; Craig, Teresa; Myers, John G; Durante, William; Stewart, Ronald M; Johnson, Fruzsina K.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 70, No. 2, 02.2011, p. 466-471.

Research output: Contribution to journalArticle

Nicholson, Susannah E. ; Johnson, Robert A. ; Craig, Teresa ; Myers, John G ; Durante, William ; Stewart, Ronald M ; Johnson, Fruzsina K. / Transfusion-related acute lung injury in a rat model of trauma-hemorrhage. In: Journal of Trauma - Injury, Infection and Critical Care. 2011 ; Vol. 70, No. 2. pp. 466-471.
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