Transfection of cells mediated by biodegradable polymer materials with surface-bound polyethyleneimine

Ji Zheng, William S. Manuel, Peter J. Hornsby

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Poly(ε-CBZ-L-lysine) can be mixed with biodegradable polymers such as poly(D,L-lactic-co-glycolic acid) or poly(L-lactic acid) and formed into films, foams, or microspheres. Surface amino groups may then be deprotected with acid or lithium/liquid ammonia. The amino groups serve as a method to modify the surface by attachment of other molecules. In the present experiments, we show that these polymer materials, as films or foams, may be surface modified by the attachment of polyethyleneimine (PEI). Plasmid DNA attached to the PEI can transfect cells plated on the surface over several days. Covalent attachment of PEI was required for transfection to be efficient. PEI was also attached to surface-bound collagen on cell culture plates and was shown to mediate transfection.

Original languageEnglish (US)
Pages (from-to)254-257
Number of pages4
JournalBiotechnology Progress
Volume16
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology

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