Transfection of cells mediated by biodegradable polymer materials with surface-bound polyethyleneimine

Ji Zheng, William S. Manuel, Peter J Hornsby

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Poly(ε-CBZ-L-lysine) can be mixed with biodegradable polymers such as poly(D,L-lactic-co-glycolic acid) or poly(L-lactic acid) and formed into films, foams, or microspheres. Surface amino groups may then be deprotected with acid or lithium/liquid ammonia. The amino groups serve as a method to modify the surface by attachment of other molecules. In the present experiments, we show that these polymer materials, as films or foams, may be surface modified by the attachment of polyethyleneimine (PEI). Plasmid DNA attached to the PEI can transfect cells plated on the surface over several days. Covalent attachment of PEI was required for transfection to be efficient. PEI was also attached to surface-bound collagen on cell culture plates and was shown to mediate transfection.

Original languageEnglish (US)
Pages (from-to)254-257
Number of pages4
JournalBiotechnology Progress
Volume16
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Polyethyleneimine
biodegradability
transfection
Transfection
polymers
Polymers
foams
films (materials)
cells
glycolic acid
Microspheres
Lithium
Ammonia
lithium
Lysine
Plasmids
Collagen
Cell Culture Techniques
collagen
lysine

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Transfection of cells mediated by biodegradable polymer materials with surface-bound polyethyleneimine. / Zheng, Ji; Manuel, William S.; Hornsby, Peter J.

In: Biotechnology Progress, Vol. 16, No. 2, 2000, p. 254-257.

Research output: Contribution to journalArticle

Zheng, Ji ; Manuel, William S. ; Hornsby, Peter J. / Transfection of cells mediated by biodegradable polymer materials with surface-bound polyethyleneimine. In: Biotechnology Progress. 2000 ; Vol. 16, No. 2. pp. 254-257.
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