Transepithelial transport characteristics of the antihypertensive peptide, lys-val-leu-pro-val-pro, in human intestinal caco-2 cell monolayers

Haiyan Sun, Dong Liu, Shimin Li, Zhenyu Qin

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

An antihypertensive peptide, Lys-Val-Leu-Pro-Val- Pro (KVLPVP), can reduce blood pressure in hypertensive rats after being orally administered. In this study, the transepithelial transport of intact KVLPVP was examined by Caco-2 monolayers. The results were as follows: (i) The flux was not saturable for apical (AP) to basolateral (BL) or BL-AP transport when the concentration of KVLPVP was 1-8mM. (ii) Sodium deoxycholate loosened the tight junction in the Caco-2 cells and significantly improved the transport process. (iii) Phenylarsine oxide, a transcytotic process inhibitor, had little effect on the transport process. (iv) The influx and eflux of KVLPVP remained unchanged in the presence of the ATP inhibitor sodium azide. (v) This transport was not inhibited by the peptide transporter substrates Gly-Pro or arphanine A. All these data indicate that paracellular transport diffusion was the major flux mechanism for the intact KVLPVP.

Original languageEnglish (US)
Pages (from-to)293-298
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number2
DOIs
StatePublished - Apr 30 2009

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Keywords

  • Antihypertensive peptide
  • Caco-2 cell
  • High-performance liquid chromatography (HPLC)
  • Lys-Val-Leu-Pro-Val-Pro
  • Transepithelial transport

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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