Transcriptomic epidemiology of smoking: The effect of smoking on gene expression in lymphocytes

Jac C. Charlesworth, Joanne E. Curran, Matthew P. Johnson, Harald H H Göring, Thomas D. Dyer, Vincent P. Diego, Jack W. Kent, Michael C. Mahaney, Laura Almasy, Jean W. MacCluer, Eric K. Moses, John Blangero

Research output: Contribution to journalArticle

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Abstract

Background. This investigation offers insights into system-wide pathological processes induced in response to cigarette smoke exposure by determining its influences at the gene expression level. Methods. We obtained genome-wide quantitative transcriptional profiles from 1,240 individuals from the San Antonio Family Heart Study, including 297 current smokers. Using lymphocyte samples, we identified 20,413 transcripts with significantly detectable expression levels, including both known and predicted genes. Correlation between smoking and gene expression levels was determined using a regression model that allows for residual genetic effects. Results. With a conservative false-discovery rate of 5% we identified 323 unique genes (342 transcripts) whose expression levels were significantly correlated with smoking behavior. These genes showed significant over-representation within a range of functional categories that correspond well with known smoking-related pathologies, including immune response, cell death, cancer, natural killer cell signaling and xenobiotic metabolism. Conclusions. Our results indicate that not only individual genes but entire networks of gene interaction are influenced by cigarette smoking. This is the largest in vivo transcriptomic epidemiological study of smoking to date and reveals the significant and comprehensive influence of cigarette smoke, as an environmental variable, on the expression of genes. The central importance of this manuscript is to provide a summary of the relationships between gene expression and smoking in this exceptionally large cross-sectional data set.

Original languageEnglish (US)
Article number29
JournalBMC Medical Genomics
Volume3
DOIs
StatePublished - 2010
Externally publishedYes

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Epidemiology
Smoking
Lymphocytes
Gene Expression
Smoke
Tobacco Products
Genes
Gene Regulatory Networks
Xenobiotics
Pathologic Processes
Natural Killer Cells
Epidemiologic Studies
Cell Death
Genome
Pathology
Neoplasms

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Charlesworth, J. C., Curran, J. E., Johnson, M. P., Göring, H. H. H., Dyer, T. D., Diego, V. P., ... Blangero, J. (2010). Transcriptomic epidemiology of smoking: The effect of smoking on gene expression in lymphocytes. BMC Medical Genomics, 3, [29]. https://doi.org/10.1186/1755-8794-3-29

Transcriptomic epidemiology of smoking : The effect of smoking on gene expression in lymphocytes. / Charlesworth, Jac C.; Curran, Joanne E.; Johnson, Matthew P.; Göring, Harald H H; Dyer, Thomas D.; Diego, Vincent P.; Kent, Jack W.; Mahaney, Michael C.; Almasy, Laura; MacCluer, Jean W.; Moses, Eric K.; Blangero, John.

In: BMC Medical Genomics, Vol. 3, 29, 2010.

Research output: Contribution to journalArticle

Charlesworth, JC, Curran, JE, Johnson, MP, Göring, HHH, Dyer, TD, Diego, VP, Kent, JW, Mahaney, MC, Almasy, L, MacCluer, JW, Moses, EK & Blangero, J 2010, 'Transcriptomic epidemiology of smoking: The effect of smoking on gene expression in lymphocytes', BMC Medical Genomics, vol. 3, 29. https://doi.org/10.1186/1755-8794-3-29
Charlesworth, Jac C. ; Curran, Joanne E. ; Johnson, Matthew P. ; Göring, Harald H H ; Dyer, Thomas D. ; Diego, Vincent P. ; Kent, Jack W. ; Mahaney, Michael C. ; Almasy, Laura ; MacCluer, Jean W. ; Moses, Eric K. ; Blangero, John. / Transcriptomic epidemiology of smoking : The effect of smoking on gene expression in lymphocytes. In: BMC Medical Genomics. 2010 ; Vol. 3.
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