Transcriptoma en mexicanos. Metodología para analizar el perfil de expresión genética de gran escala en muestras simultáneas de tejido muscular, adiposo y linfocitos obtenidas en un mismo individuo

Translated title of the contribution: Transcriptome in mexicans. Methodology for analyzing gene expression profile of large scale simultaneous samples of muscle tissue, adipose and lymphocytes obtained from the same individual

Raúl A. Bastarrachea, Juan Carlos López-Alvarenga, Jack W. Kent, Hugo A. Laviada-Molina, Ricardo M. Cerda-Flores, Ana Laura Calderón-Garcidueñas, Amanda Torres-Salazar, Esther C. Gallegos-Cabrales, M. Elizabeth Tejero, Shelley A. Cole, Anthony G. Comuzzie

    Research output: Contribution to journalArticle

    2 Scopus citations

    Abstract

    Objective: We describe the methodology used to analyze multiple transcripts using microarray techniques in simultaneous biopsies of muscle, adipose tissue and lymphocytes obtained from the same individual as part of the standard protocol of the Genetics of Mexican Metabolic Disorders (GMMD) Family Study. Methods: We recruited 4 healthy male subjects with BMI 20-41, who signed an informed consent letter. Subjects participated in a clinical examination that included anthropometric and body composition measurements, muscle biopsies (vasus lateralis) subcutaneous fat, and a blood draw. All samples provided sufficient amplified RNA for microarray analysis. Total RNA was extracted from the biopsy samples and amplified for analysis. Results: Of the 48 687 transcript targets queried, 39.4% were detectable in a least one of the studied tissues. Leptin was not detectable in lymphocytes, weakly expressed in muscle, but overexpressed and highly correlated with BMI in subcutaneous fat. Another example was GLUT4, which was detectable only in muscle and not correlated with BMI. Expression level concordance was 0.7 (p<0.001) for the three tissues studied. Conclusions: We demonstrated the feasibility of carrying out simultaneous analysis of gene expression in multiple tissues, concordance of genetic expression in different tissues, and confidence that this method corroborates the expected biological relationships among LEP and GLUT4. The GEMM study will provide a broad and valuable overview on metabolic diseases, including obesity and type 2 diabetes.

    Original languageSpanish
    Pages (from-to)473-479
    Number of pages7
    JournalGaceta Medica de Mexico
    Volume144
    Issue number6
    Publication statusPublished - Nov 1 2008

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    Keywords

    • Genetic expression
    • Messenger RNA
    • Metabolic syndrome (syndrome X)
    • Microarray analysis
    • Untranslated RNA

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Bastarrachea, R. A., López-Alvarenga, J. C., Kent, J. W., Laviada-Molina, H. A., Cerda-Flores, R. M., Calderón-Garcidueñas, A. L., ... Comuzzie, A. G. (2008). Transcriptoma en mexicanos. Metodología para analizar el perfil de expresión genética de gran escala en muestras simultáneas de tejido muscular, adiposo y linfocitos obtenidas en un mismo individuo. Gaceta Medica de Mexico, 144(6), 473-479.