Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1

Ceri Batchelder, Melanie A. Dunn, Bob Choy, Yong Suh, Conrad Cassie, Eun Yong Shim, Tae Ho Shin, Craig Mello, Geraldine Seydoux, T. Keith Blackwell

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87 Scopus citations

Abstract

In the early Caenorhabditis elegans embryo, maternally expressed PIE-1 protein is required in germ-line blastomeres to inhibit somatic differentiation, maintain an absence of mRNA transcription, and block phosphorylation of the RNA polymerase II large subunit (Pol II) carboxy- terminal domain (CTD). We have determined that PIE-1 can function as a transcriptional repressor in cell culture assays. By fusing PIE-1 sequences to the yeast GAL4 DNA-binding domain, we have identified a PIE-1 repression domain that appears to inhibit the transcriptional machinery directly. A sequence element that is required for this repressor activity is similar to the Pol II CTD heptapeptide repeat, suggesting that the PIE-1 repression domain might target a protein complex that can bind the CTD. An alteration of this sequence element that blocks repression also impairs the ability of a transgene to rescue a pie-1 mutation, suggesting that this repressor activity may be important for PIE-1 function in vivo.

Original languageEnglish (US)
Pages (from-to)202-212
Number of pages11
JournalGenes and Development
Volume13
Issue number2
DOIs
StatePublished - Jan 15 1999

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Keywords

  • C. elegans
  • Germ line
  • RNA Pol II CTD
  • Repression
  • Transcription
  • Zinc finger

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Batchelder, C., Dunn, M. A., Choy, B., Suh, Y., Cassie, C., Shim, E. Y., Shin, T. H., Mello, C., Seydoux, G., & Blackwell, T. K. (1999). Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1. Genes and Development, 13(2), 202-212. https://doi.org/10.1101/gad.13.2.202